Development of an experimental ovarian tumor: immunocytochemical analysis

Autor: C. Libertun, Stephanie Fritz, C. Beyer, Dale B. Hales, Eleonora Sorianello, Artur Mayerhofer, V. Lux-Lantos
Rok vydání: 2002
Předmět:
Time Factors
Endocrinology
Diabetes and Metabolism
AROMATASE
Apoptosis
OVARIAN TUMOR
Rats
Sprague-Dawley
purl.org/becyt/ford/1 [https]
Ovarian tumor
Endocrinology
Follicular phase
Aromatase
Ovarian Neoplasms
biology
Luteoma
Reverse Transcriptase Polymerase Chain Reaction
Steroidogenic acute regulatory protein
Patología
purl.org/becyt/ford/3.1 [https]
General Medicine
Bioquímica y Biología Molecular
Immunohistochemistry
Cx43
Medicina Básica
medicine.anatomical_structure
Female
purl.org/becyt/ford/3 [https]
Gonadotropin
Cell Division
CIENCIAS NATURALES Y EXACTAS
endocrine system
medicine.medical_specialty
StAR
CIENCIAS MÉDICAS Y DE LA SALUD
Synaptosomal-Associated Protein 25
Tyrosine 3-Monooxygenase
medicine.drug_class
Ovariectomy
Immunocytochemistry
Nerve Tissue Proteins
Ovary
Ciencias Biológicas
Proliferating Cell Nuclear Antigen
Internal medicine
In Situ Nick-End Labeling
medicine
Animals
PCNA
RNA
Messenger
IMMUNOHISTOCHEMISTRY
purl.org/becyt/ford/1.6 [https]
Membrane Proteins
Blotting
Northern
Phosphoproteins
medicine.disease
Rats
Disease Models
Animal
Connexin 43
SNAP-25
biology.protein
Spleen
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
ISSN: 1479-683X
0804-4643
DOI: 10.1530/eje.0.1470387
Popis: OBJECTIVE: The aim of the present work was to study whether immunocytochemical parameters present in the normal ovary were altered after tumor development under high gonadotropin levels. METHODS: Ovarian tumors (luteoma): castrated female rats had an ovary grafted into the spleen; tumors were left to develop for 1, 2, 3 or 7 months. The presence of apoptotic cells (TUNEL method) and the expression of proliferating cell nuclear antigen (PCNA), gap junction protein (Cx43), steroidogenic acute regulatory protein (StAR), aromatase and synaptosome-associated protein of 25 kDa (SNAP-25) were determined by immunocytochemistry. Some of these findings were confirmed by RT-PCR (Cx43, StAR, SNAP-25). Inhibin subunit mRNAs were investigated by Northern blot. RESULTS: PCNA staining of tumors was mainly found in granulosa cells of transforming follicles and was absent from luteinized follicles. A nearly complete absence of apoptosis was observed. Cx43 was mainly found in follicles, while it was very weakly expressed or absent in luteinized follicles. StAR protein expression, indicating active steroidogenesis, was demonstrated only in luteinized follicles and in thecal cells, but was absent from granulosa cells. Aromatase immunoreactivity was very intense in granulosa and also present in luteal cells. Membrane-associated and cytoplasmic SNAP-25 immunostaining was determined in patches of endocrine cells in the follicles, as well as in the luteinized follicles. The expression of mRNAs for Cx43, StAR and SNAP-25 (RT-PCR) and inhibin subunits (Northern blots) were confirmed in 1-, 3- and 7-month-old tumors. CONCLUSIONS: These results indicated that luteoma most likely develop from unruptured follicles by hypertrophy and proliferation of follicular cells. Circulating gonadotropins seem to play a fundamental role in maintaining the expression of proteins typically expressed in normal ovary, while avoiding apoptosis in this tissue.
Databáze: OpenAIRE
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