MKL-1 regulates the stem cell marker. CD44 in breast cancer cells
| Autor: | Zhou-Tong Dai, Ao Yao, Yuan Xiang, Jia Peng Li, Wei Guo, Hui-Min Zhang, Feng Huang, Han-Han Li, Zi-Jiang Zhang, Hui Li, Xing Hua Liao |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
lcsh:GE1-350
0301 basic medicine Regulation of gene expression biology CD44 Promoter Stem cell marker Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Gene expression biology.protein Stem cell Signal transduction Gene lcsh:Environmental sciences |
| Zdroj: | E3S Web of Conferences, Vol 78, p 01002 (2019) |
| ISSN: | 2267-1242 |
| DOI: | 10.1051/e3sconf/20197801002 |
| Popis: | CD44, cluster of differentiation 44 is a typical marker of stem cells. At present, it has been found that CD44 is prevalent in various human malignant tumors, but its expression regulation mechanism is still not clear. The initiation of gene expression, the modification of RNA levels, and the regulation of protein levels are the main factors affecting the expression level of genes, and the most critical one is the regulation of gene expression by signaling pathways. Up to now, there has been no report on the role of MKL-1 in the cloning of the cd44 promoter. Therefore, this study intends to clone the cd44 gene promoter, construct its luciferase reporter gene vector, transfect the MKL-1 overexpression vector, and analyze how it affects transcriptional activity, in order to further study the expression regulation of cd44. The mechanism provides a powerful tool in the future. |
| Databáze: | OpenAIRE |
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