Molecular population genetic analysis of a Streptococcus pyogenes bacteriophage-encoded hyaluronidase gene: recombination contributes to allelic variation
| Autor: | Vivek Kapur, Ann Marie Marciel, James M. Musser |
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| Rok vydání: | 1997 |
| Předmět: |
Streptococcus Phages
Streptococcus pyogenes Population Molecular Sequence Data Hyaluronoglucosaminidase medicine.disease_cause Microbiology Genetic analysis Genetic recombination Bacteriophage Viral Proteins Hyaluronidase medicine Humans Allele education Gene Alleles Genetics Recombination Genetic education.field_of_study biology Base Sequence Genetic Variation Sequence Analysis DNA biology.organism_classification Infectious Diseases DNA Viral Collagen medicine.drug |
| Zdroj: | Microbial pathogenesis. 22(4) |
| ISSN: | 0882-4010 |
| Popis: | Many strains of the human pathogenic bacterium Streptococcus pyogenes produce hyaluronidase, an enzyme that degrades hyaluronic acid, a major component of the extracellular matrix. Degradation of hyaluronic acid is thought to aid in host tissue invasion and dissemination of S. pyogenes . The molecular population genetics of the bacteriophage-encoded hyaluronidase gene ( hyl ) was analysed by sequencing the gene from 13 streptococcal strains representing seven well-differentiated multilocus enzyme electrophoretic types and eight M or T protein serotypes. Substantial levels of allelic polymorphism were identified, and the analysis found strong statistical evidence that recombinational processes have contributed to the generation of molecular variation in this gene. A 111 base pair segment of hyl encoding a collagenous motif, that may bind collagen, was absent in a serotype M14 isolate and 13 serotype M18 multilocus enzyme electrophoretic type 20 strains examined. The analysis provides a molecular population genetics framework for studies examining the role of naturally occurring hyaluronidase variation in host–pathogen interactions. |
| Databáze: | OpenAIRE |
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