Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg)
| Autor: | Cristina Paveto, María C. Güida, Héctor N. Torres, Alejandra M. Camino, Mirtha M. Flawiá, Mónica I. Esteva |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Programmed cell death Trypanosoma cruzi Immunology DNA Fragmentation Parasitemia Epigallocatechin gallate Pharmacology complex mixtures Antioxidants Catechin Mice Random Allocation chemistry.chemical_compound In vivo In Situ Nick-End Labeling medicine Animals Chagas Disease heterocyclic compounds Fragmentation (cell biology) Mice Inbred BALB C Dose-Response Relationship Drug biology food and beverages General Medicine medicine.disease biology.organism_classification Trypanocidal Agents In vitro Disease Models Animal Dose–response relationship Infectious Diseases chemistry Biochemistry Hepatocytes Parasitology sense organs |
| Zdroj: | Experimental Parasitology. 117:188-194 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2007.04.015 |
| Popis: | The trypanocidal activity of catechins on Trypanosoma cruzi bloodstream trypomastigotes has been previously reported. Herein, we present the effect of epigallocatechin gallate (EGCg) on parasitemia and survival in a murine model of acute Chagas' disease as well as on the epimastigote form of the parasite. Upon intraperitoneal administration of daily doses of 0.8 mg/kg/day of EGCg for 45 days, mice survival rates increased from 11% to 60%, while parasitemia diminished to 50%. No side effects were observed in EGCg-treated animals. Fifty percent inhibition of epimastigotes growth was achieved with 311 microM EGCg 120 h after drug addition. No lysis, total culture growth inhibition or morphological changes were observed upon addition of 1-3mM EGCg at 24 h. This treatment also produced oligosomal fragmentation of epimastigotes DNA, suggesting a programmed cell death (PCD)-like process. All these findings point out EGCg as a potential new lead compound for chemotherapy of Chagas' disease. |
| Databáze: | OpenAIRE |
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