Influence of oxygen transfer on Pseudomonas putida effects on growth rate and biodesulfurization capacity
| Autor: | A. Alcon, Felix Garcia-Ochoa, Emilio Miraflores Gómez, Sebastian Escobar, Victoria E. Santos, Alberto Rodríguez |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Oxygen transfer Bioengineering 01 natural sciences Mixed Function Oxygenases 03 medical and health sciences Bioreactors Bacterial Proteins 010608 biotechnology Bioreactor Growth rate chemistry.chemical_classification biology Pseudomonas putida Chemistry General Medicine Monooxygenase equipment and supplies biology.organism_classification Flue-gas desulfurization Oxygen 030104 developmental biology Enzyme Chemical engineering Industrial and production engineering Biotechnology |
| Zdroj: | Bioprocess and Biosystems Engineering. 39:545-554 |
| ISSN: | 1615-7605 1615-7591 |
| Popis: | The growth rate and desulfurization capacity accumulated by the cells during the growth of Pseudomonas putida KTH2 under different oxygen transfer conditions in a stirred and sparged tank bioreactor have been studied. Hydrodynamic conditions were changed using different agitation conditions. During the culture, several magnitudes associated to growth, such as the specific growth rate, the dissolved oxygen concentration and the carbon source consumption have been measured. Experimental results indicate that cultures are influenced by the fluid dynamic conditions into the bioreactor. An increase in the stirrer speed from 400 to 700 rpm has a positive influence on the cell growth rate. Nevertheless, the increase of agitation from 700 to 2000 rpm hardly has any influence on the growth rate. The effect of fluid dynamics on the cells development of the biodesulfurization (BDS) capacity of the cells during growth is different. The activities of the intracellular enzymes involved in the 4S pathway change with dissolved oxygen concentration. The enzyme activities have been evaluated in cells at several growth time and different hydrodynamic conditions. An increase of the agitation from 100 to 300 rpm has a positive influence on the development of the overall BDS capacity of the cells during growth. This capacity shows a decrease for higher stirrer speeds and the activity of the enzymes monooxygenases DszC and DszA decreases dramatically. The highest value of the activity of DszB enzyme was obtained with cells cultured at 100 rpm, while this activity decreases when the stirrer speed was increased higher than this value. |
| Databáze: | OpenAIRE |
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