Sperm aging in utero and chromosomal anomalies in rabbit blastocysts
| Autor: | Evelyn L. Shaver, Patricia A. Martin |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Male
Ovulation endocrine system Time Factors Chromosome Disorders Digyny Biology Insemination Andrology Recovery rate Corpus Luteum Animals Molecular Biology Sex Chromosome Aberrations reproductive and urinary physiology Chromosome Aberrations Mosaicism urogenital system Significant difference Cell Biology Anatomy Embryo Mammalian Spermatozoa Sperm In utero Karyotyping Female Rabbits Sex ratio Developmental Biology |
| Zdroj: | Developmental Biology. 28:480-486 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/0012-1606(72)90031-0 |
| Popis: | Chromosomes were studied in 239 rabbit blastocysts recovered after aging of spermatozoa in utero . Mature females were inseminated artificially and induced to ovulate with an injection of HCG. The administration of HCG was delayed 4–21 hr after insemination, thus allowing sperm to age. Six-day blastocysts were recovered from females up to an estimated 30 hr sperm aging. There was a peak in the recovery rate at 18 hr and a decline thereafter. A significant difference was found between the incidences of chromosomal anomalies in the control and experimental series, where they were 1% and 9.7%, respectively. The abnormalities included mosaics, mixoploids, and XX/XY chimeras. The latter has been hitherto unreported in the rabbit. Dispermy with accompanying digyny has been implicated as the possible mode of origin of the chimeras. The sex ratio was not affected significantly by aging of spermatozoa. |
| Databáze: | OpenAIRE |
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