Poxytrins, a class of oxygenated products from polyunsaturated fatty acids, potently inhibit blood platelet aggregation

Autor: Evelyne Véricel, Michel Lagarde, Michel Guichardant, Ping Chen
Přispěvatelé: Régulations métaboliques, nutrition et diabètes (RMND), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), INSERM, Ministry of Education and Research, Lipides pour l'Industrie et la Sante (LISA) Carnot Institute, Chinese Educational Council
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Thromboxane
Receptors
Thromboxane
Protectin D1
Biochemistry
chemistry.chemical_compound
Lipoxygenase
15-lipoxygenase
MESH: Collagen
Neuroprotectin
Cyclic AMP
Dicarboxylic Acids
thromboxane receptor
MESH: Blood Platelets
MESH: Cyclic AMP
MESH: Platelet Aggregation
chemistry.chemical_classification
Arachidonic Acid
biology
Chemistry
Thromboxanes
MESH: Fatty Acids
Unsaturated
docosahexaenoic acid
docosatrienes
MESH: Receptors
Thromboxane
MESH: Docosahexaenoic Acids
Docosahexaenoic acid
platelet aggregation
MESH: Platelet Aggregation Inhibitors
Fatty Acids
Unsaturated
Platelet aggregation inhibitor
Arachidonic acid
Collagen
MESH: Oxygen
Biotechnology
Polyunsaturated fatty acid
Blood Platelets
Docosahexaenoic Acids
MESH: Prostaglandin-Endoperoxide Synthases
MESH: Thromboxanes
MESH: Cyclooxygenase Inhibitors
Isomerism
MESH: Dicarboxylic Acids
Genetics
Humans
MESH: Isomerism
Cyclooxygenase Inhibitors
Molecular Biology
MESH: Humans
MESH: 15-Hydroxy-11 alpha
9 alpha-(epoxymethano)prosta-5
13-dienoic Acid
MESH: Arachidonic Acid
Oxygen
Prostaglandin-Endoperoxide Synthases
15-Hydroxy-11 alpha
9 alpha-(epoxymethano)prosta-5
13-dienoic Acid
biology.protein
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Platelet Aggregation Inhibitors
Zdroj: FASEB Journal
FASEB Journal, Federation of American Society of Experimental Biology, 2011, 25 (1), pp.382-8. ⟨10.1096/fj.10-161836⟩
ISSN: 0892-6638
1530-6860
DOI: 10.1096/fj.10-161836⟩
Popis: International audience; Docosahexaenoic acid (DHA), an important component of marine lipids, exhibits anti-inflammatory activity related to some of its oxygenated metabolites, such as neuroprotectin/protectin D1 [NPD1/PD1; 10(R),17(S)-dihydroxy-docosa-4Z,7Z, 11E,13E,15Z,19Z-hexaenoic acid] produced through the 15-lipoxygenase pathway. However, other metabolites from DHA can be produced through this pathway, and other polyunsaturated fatty acids (PUFAs) of nutritional value may be oxygenated as well. Their biological activities remain unknown. Isomers of protectin D1 were synthesized using soybean lipoxygenase and tested for their ability to inhibit human blood platelet aggregation. A geometric isomer called PDX, previously described with the 11E,13Z,15E geometry, instead of 11E,13E,15Z in PD1, inhibited platelet aggregation at submicromolar concentrations when induced by either collagen, arachidonic acid, or thromboxane. The inhibition occurred at the level of both the cyclooxygenase activity and thromboxane receptor site. Interestingly, all the metabolites tested exhibiting the E,Z,E-conjugated triene were active, whereas E,E,Z trienes (as in PD1) or all-trans (E,E,E) trienes were inactive. We conclude that PDX and other oxygenated products from PUFAs of nutritional interest, having the E,Z,E-conjugated triene motif and collectively named poxytrins (PUFA oxygenated trienes), might have antithrombotic potential.
Databáze: OpenAIRE
Externí odkaz: