Luteinizing Hormone-Releasing Hormone and Thyrotropin-Releasing Hormone in Human and Bovine Milk
| Autor: | Mati Fridkin, Yitzhak Koch, Tanchum Amarant |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Bovine milk Radioimmunoassay Thyrotropin-releasing hormone Peptide hormone Biochemistry High-performance liquid chromatography Gonadotropin-Releasing Hormone fluids and secretions Internal medicine medicine Animals Humans Thyrotropin-Releasing Hormone Chromatography High Pressure Liquid Milk Human Chemistry food and beverages Rats Inbred Strains Rats Milk Endocrinology Colostrum Cattle Female Luteinizing hormone hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | European Journal of Biochemistry. 127:647-650 |
| ISSN: | 1432-1033 0014-2956 |
| Popis: | Two hypothalamic peptide hormones, luteinizing hormone-releasing hormone (LHRH) and thyrotropin-releasing hormone (TRH), have been isolated from human milk and bovine colostrum. Acidified methanolic extracts, prepared from human milk, bovine colostrum and rat hypothalami, as well as synthetic LHRH and TRH markers were subjected to high-pressure liquid chromatography (HPLC). The eluates were tested for the presence of LHRH and TRH by specific radioimmunoassays. It was found that milk extracts contain significant amounts of LHRH (3.9 - 11.8 ng/ml) and TRH (0.16 - 0.34 ng/ml), which comigrate with the corresponding marker hormones and with those of hypothalamic origin. The HPLC-purified LHRH from both human and bovine milk was bioactive in a dose-response manner similar to synthetic LHRH. |
| Databáze: | OpenAIRE |
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