Topical Anti-Inflammatory and Analgesic Activities of Citrullus colocynthis Extract Cream in Rats
| Autor: | Hassan Rajabi Vardanjani, Hossein Rajabi Vardanjani, Marzieh Pashmforosh, Mohammad Javad Khodayar, Mahdi Pashmforosh |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Medicine (General) medicine.drug_class Analgesic (+)-Naloxone Pharmacology Anti-inflammatory cream 03 medical and health sciences R5-920 0302 clinical medicine Edema medicine rat skin and connective tissue diseases anti-inflammatory Hydrocortisone business.industry food and beverages General Medicine antinociceptive 030104 developmental biology Nociception Opioid Citrullus colocynthis medicine.symptom business 030217 neurology & neurosurgery Opioid antagonist medicine.drug |
| Zdroj: | Medicina; Volume 54; Issue 4; Pages: 51 Medicina, Vol 54, Iss 4, p 51 (2018) Medicina Volume 54 Issue 4 |
| ISSN: | 1648-9144 |
| DOI: | 10.3390/medicina54040051 |
| Popis: | Background and objectives: Citrullus colocynthis (CC), known as bitter apple, is used to treat diabetes in Iranian traditional medicine. The aim of this study is to evaluate the anti-inflammatory and analgesic activities of CC cream in rats. Materials and Methods: The carrageenan-induced edema in a rat hind paw was carried out to evaluate the topical anti-inflammatory effect of the CC fruit extract cream (2&ndash 8%) and the tissue levels of IL-6 and TNF-&alpha were estimated by using a commercial ELISA kit. The topical antinociceptive activity of CC cream (2&ndash 8%) was evaluated in the rat formalin test. To determine the role of opioid receptors in the local antinociceptive effect of the CC cream, naloxone (20 &mu g/paw, i.pl.), a non-selective opioid antagonist, was used. Results: The results showed that the CC cream (2&ndash 8%) dose-dependently reduced the carrageenan-induced paw edema and reversed the changes in the level of TNF-&alpha and IL-6 due to carrageenan-induced edema (p < 0.01). The anti-inflammatory effect of CC cream 8% was comparable to that of hydrocortisone ointment 1%. Furthermore, the application of CC cream (2&ndash 8%) dose-dependently inhibited both first and second phases of the formalin test (p < 0.05). The antinociceptive effect of the CC cream (8%) was comparable to that of methyl salicylate cream 30%. Moreover, the administration of naloxone significantly reversed the topical antinociceptive effect of the CC cream (p < 0.05). Conclusions: For the first time, this study indicated that the topical application of CC cream possesses significant anti-inflammatory and antinociceptive activities in animal models, which were probably mediated by opioid receptors and the suppression of pro-inflammatory cytokines (TNF-&alpha and IL-6). Thus, the CC cream can be used to treat inflammatory pain and inflammatory diseases. |
| Databáze: | OpenAIRE |
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