Elimination of adhering bacteria from surfaces by pulsed laser beams
| Autor: | A.-K. Sadoudi, Olivier Cerf, Jean-Marie Herry |
|---|---|
| Přispěvatelé: | Bioadhésion et Hygiène des Matériaux (BHM), Institut National de la Recherche Agronomique (INRA)-AgroParisTech |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Pulsed laser
Materials science Colony Count Microbial Analytical chemistry 02 engineering and technology 01 natural sciences Applied Microbiology and Biotechnology 010305 fluids & plasmas Microbiology 0103 physical sciences Escherichia coli Laser beams ComputingMilieux_MISCELLANEOUS biology Lasers Human decontamination Stainless Steel 021001 nanoscience & nanotechnology biology.organism_classification Disinfection [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Biofilms Food Microbiology Energy density 0210 nano-technology Bacteria |
| Zdroj: | Letters in Applied Microbiology Letters in Applied Microbiology, Wiley, 1997, 24, pp.177-179 |
| ISSN: | 0266-8254 1472-765X |
| Popis: | As an alternative to the use of chemicals for cleaning and disinfecting surfaces of equipment in food industry, the efficacy of pulsed laser beams for removal and killing of adherent bacteria from stainless steel surfaces was assessed. Escherichia coli biofilms were produced under dynamic conditions in diluted nutritive medium incubated at 37 degrees C for 24 h. Influence of energy density and number of shots were tested at three wavelengths (1064, 532 and 355 nm). With one 20 ns pulse, results range from 3.5 decimal reductions of the microbial load with < or = 50 MW cm-2 without visible alteration of the surface, to more than 6 decimal reductions with < or = 600 MW cm-2. The measured effect was largely attributed to removal of the micro-organisms and transfer to the surrounding air. The treatment could therefore be improved with respect to the numbers remaining associated with the surface by venting. |
| Databáze: | OpenAIRE |
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