Tick salivary gland extracts promote virus growth in vitro
| Autor: | Mirko Slovák, N. Fuchsberger, Milan Labuda, P. Kocakova, Patricia A. Nuttall, Valéria Hajnická |
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| Rok vydání: | 1998 |
| Předmět: |
Saliva
Time Factors viruses In Vitro Techniques Salivary Glands Vesicular stomatitis Indiana virus Virus Mice L Cells Ticks In vivo medicine Animals Mononegavirales Connective Tissue Cells biology Salivary gland Tissue Extracts Viral Core Proteins Rhabdoviridae biology.organism_classification Virology In vitro Infectious Diseases medicine.anatomical_structure Vesicular stomatitis virus Electrophoresis Polyacrylamide Gel Animal Science and Zoology Parasitology |
| Zdroj: | Parasitology. 116:533-538 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182098002686 |
| Popis: | Saliva of blood-feeding arthropods promotes infection by the vector-borne pathogens they transmit. To investigate this phenomenon in vitro, cultures of mouse L cells were treated with a salivary gland extract (SGE) prepared from feeding ticks and then infected with vesicular stomatitis virus (VSV). At low input doses of VSV, viral yield was increased 100-fold to 10000-fold by 16–23 h post-infection compared with untreated cultures, and depending on the SGE concentration. SGE-mediated acceleration of viral yield corresponded with the earlier appearance of VSV nucleocapsid protein as detected by 2-dimensional electrophoresis of infected cells. The observation that physiological doses of virus (i.e. doses likely to be inoculated by an infected arthropod vector into its vertebrate host during blood-feeding) respond to SGE treatment in vitro provides a new opportunity for identifying the factors in tick saliva that promote virus transmission in vivo. |
| Databáze: | OpenAIRE |
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