Modulation of the CD4+ T cell response after acellular pertussis vaccination in the presence of TLR4 ligation
| Autor: | Brummelman, Jolanda, Helm, Kina, Hamstra, Hendrik-Jan, van der Ley, Peter, Boog, Claire J P, Han, Wanda G H, van Els, Cécile A C M, LS Immunologie, dI&I RA-I&I I&I, Infection & Immunity, Risk Assessment |
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| Přispěvatelé: | LS Immunologie, dI&I RA-I&I I&I, Infection & Immunity, Risk Assessment |
| Rok vydání: | 2015 |
| Předmět: |
CD4-Positive T-Lymphocytes
Bordetella pertussis medicine.medical_treatment T cell Immunization Secondary CD4 T cells Diphtheria-Tetanus-acellular Pertussis Vaccines Memory T cells Mice Th2 Cells Adjuvants Immunologic Immunity Animals Medicine Adjuvants TLR4 Acellular pertussis vaccine MHC class II General Veterinary General Immunology and Microbiology biology business.industry Public Health Environmental and Occupational Health Th1 Cells biology.organism_classification Toll-Like Receptor 4 Vaccination Phenotype Infectious Diseases medicine.anatomical_structure Immunoglobulin G Immunology biology.protein Cytokines Th17 Cells Molecular Medicine Lymph business Immunologic Memory Adjuvant |
| Zdroj: | Vaccine, 33(12), 1483. Elsevier |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2015.01.063 |
| Popis: | Whole cell pertussis (wP) vaccines are gradually being replaced by aluminum salt-adjuvanted acellular pertussis (aP) vaccines. These promote CD4(+) T cell responses with a non-protective Th2 component, while protective immune mechanisms to B. pertussis may rather involve long-lived Th1/Th17 type CD4(+) T cells. Here we asked whether addition of a non-toxic meningococcal LPS derivative, LpxL1, as adjuvant can favorably modulate the aP-induced pertussis-specific CD4(+) T cell response in mice. To assess the effect of TLR4 ligation, Th type, quantity, and memory potential of pertussis-specific CD4(+) T cells were determined at the single-cell level after aP and aP+LpxL1 vaccination using intracellular cytokine staining and MHC class II tetramers. Adding LpxL1 to the aP vaccine weakened the Th2 component and strengthened the Th1/Th17 component of the specific CD4(+) T cell response. Notably, LpxL1 addition also induced higher frequencies of tetramer positive CD4(+) T cells in draining lymph nodes or blood, depending on the phase after vaccination. Moreover, there was a net profit in the number of CD4(+) T cells with a central memory phenotype, preferred for long-term immunity. Thus, adding a TLR4 ligand as adjuvant to a current aP vaccine was associated with a more favorable pertussis-specific CD4(+) T cell response. |
| Databáze: | OpenAIRE |
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