Antimicrobial potential of egg Yolk ovoinhibitor, a multidomain Kazal-like inhibitor of chicken egg
| Autor: | Valérie Labas, Yves Nys, Gwenaelle Le Blay, S. Attucci, Joël Gautron, Magali Berges, Marie-Christine Bourin, Sophie Réhault-Godbert |
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| Přispěvatelé: | Recherches Avicoles (SRA), Institut National de la Recherche Agronomique (INRA), U618, Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM), Université de Brest (UBO), Institut Fédératif de Recherche 148 Brest - Sciences et Ingénierie en Biologie Santé (IFR 148 Brest), Université européenne de Bretagne - European University of Brittany (UEB), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), This research was supported by the French National Research Agency (OVO-mining, ANR-09-BLAN-0136) and the European Community (RESCAPE Project FOOD CT 2006-03018). M.B. acknowledges Region Centre for financial support., Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Serine Proteinase Inhibitors
food.ingredient food science and technology Molecular Sequence Data Bacillus thuringiensis Egg Proteins Dietary Biology chemistry 03 medical and health sciences food jaune d'oeuf Anti-Infective Agents antibacterial activity Yolk expression [SDV.IDA]Life Sciences [q-bio]/Food engineering medicine Animals Amino Acid Sequence Eggshell 030304 developmental biology agriculture Serine protease 0303 health sciences Yolk plasma chicken egg 030302 biochemistry & molecular biology Subtilisin General Chemistry Trypsin biology.organism_classification sexual maturation Egg Yolk 3. Good health anti-protease Biochemistry Immunology embryonic structures biology.protein ovoinhibiteur Female Trypsin Inhibitors General Agricultural and Biological Sciences Chickens medicine.drug Egg white |
| Zdroj: | Journal of Agricultural and Food Chemistry Journal of Agricultural and Food Chemistry, American Chemical Society, 2011, 59 (23), pp.12368-12374. ⟨10.1021/jf203339t⟩ |
| ISSN: | 0021-8561 1520-5118 |
| DOI: | 10.1021/jf203339t⟩ |
| Popis: | Chicken egg ovoinhibitor is a multidomain Kazal-type serine protease inhibitor with unknown function. Comparison of expression between different tissues indicated that ovoinhibitor is highly expressed in the magnum and liver followed by the uterus, which secrete egg white, egg yolk, and eggshell precursors, respectively. The results also revealed that ovoinhibitor expression is increased in the liver during sexual maturation followed by a subsequent decrease in mature hens. Ovoinhibitor was purified from the egg yolk plasma from nonfertilized eggs using two consecutive affinity chromatographies and gel filtration. Purified egg yolk ovoinhibitor was shown to inhibit trypsin and subtilisin. It was shown that purified egg yolk ovoinhibitor exhibited antimicrobial activities against Bacillus thuringiensis . The results suggest that this anti-protease plays a significant role in antibacterial egg defense against Bacillus spp., preventing contamination of table eggs (nonfertilized eggs) and protecting the chick embryo (fertilized eggs). |
| Databáze: | OpenAIRE |
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