Hormone sensitivityin vitro andin vivo of v-ras-transfected mcf-7 cell derivatives
Autor: | Guy Leclercq, F. Van Roy, Nicole Legros, Rudi Beyaert, Kris Vleminckx, N Devleeschouwer, Marcus Mareel, Walter Fiers, Carl Muquardt, M. Bracke |
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Rok vydání: | 1990 |
Předmět: |
Cancer Research
medicine.medical_specialty Cell type medicine.drug_class Cell Mice Nude Estrogen receptor Breast Neoplasms Biology Transfection Mice Internal medicine Tumor Cells Cultured medicine Animals Humans Neoplasm Invasiveness Neoplasm Metastasis Receptor Estradiol Oncogene Tamoxifen Genes ras medicine.anatomical_structure Endocrinology Receptors Estrogen Oncology Estrogen Cell culture Cancer research Cell Division Neoplasm Transplantation |
Zdroj: | International Journal of Cancer. 46:522-532 |
ISSN: | 1097-0215 0020-7136 |
DOI: | 10.1002/ijc.2910460332 |
Popis: | Human mammary carcinoma cell lines (MCF-7) were analysed for their hormone sensitivity before and after transfection with a v-Ha-ras oncogene or with a neomycin-resistance gene followed by selection in vitro or in vivo. Our aim was to test how the expression of the ras oncogene would influence the estradiol sensitivity of MCF-7 cells. In culture, MCF-7 cells expressing the viral p21 oncogene product, as compared to parental MCF-7 cells and their control derivatives, showed lower levels of a 67-kDa estrogen receptor. Progesterone receptors, however, remained sensitive to up-regulation by estrogens. The oncogene-expressing cells were less sensitive than all controls to stimulation of proliferation by 10(-8)M estradiol or to inhibition of proliferation by 2-CH3-4-OH tamoxifen, and this was not dependent upon the type of culture medium used. After s.c. or i.p. injection into female athymic nude mice, ovariectomized or left intact, the growth of MCF-7 cells expressing the ras oncogene product and of all control cells was sensitive to stimulation by estrogen supplementation. Conversely, cell lines derived from tumors generated with long latency in untreated athymic nude mice by v-ras-expressing MCF-7 cells showed efficient formation of quickly growing tumors in the absence of estrogen supplementation. No differences were observed in invasion and metastasis of the different MCF-7 cell types injected into athymic nude mice that were supplemented with estrogens or not. |
Databáze: | OpenAIRE |
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