Biosensor cell assay for measuring real-time aldosterone-induced release of histamine from mesenteric arteries
| Autor: | Kenneth Andersen, Emil Geertsen Dalgaard, Pernille B. Lærkegaard Hansen, Per Svenningsen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Calcium/metabolism Physiology histamine detection mast cells Histamine H1 receptor Biosensing Techniques 030204 cardiovascular system & hematology Biology Aldosterone/pharmacology biosensor Histamine/metabolism 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Internal medicine medicine Animals Humans Receptors Histamine H1 Aldosterone Mesenteric arteries Calcium metabolism aldosterone Transfection perfused mesenteric artery Mesenteric Arteries 030104 developmental biology Endocrinology medicine.anatomical_structure HEK293 Cells chemistry Receptors Histamine H1/metabolism Calcium Mesenteric Arteries/drug effects Histamine Intracellular Artery |
| Zdroj: | Dalgaard, E G, Andersen, K, Svenningsen, P & Hansen, P B L 2017, ' Biosensor cell assay for measuring real-time aldosterone-induced release of histamine from mesenteric arteries ', Acta Physiologica , vol. 219, no. 1, pp. 219-226 . https://doi.org/10.1111/apha.12680 Dalgaard, E G, Andersen, K, Svenningsen, P & Hansen, P B L 2017, ' Biosensor cell assay for measuring real-time aldosterone-induced release of histamine from mesenteric arteries ', Acta Physiologica, vol. 219, no. 1, pp. 219-226 . https://doi.org/10.1111/apha.12680 |
| DOI: | 10.1111/apha.12680 |
| Popis: | AIMS: The aims were to develop a method for real-time detection of histamine release and to test if incubation with aldosterone induces histamine release from isolated, perfused mice mesenteric arteries.METHODS: Fura-2 loaded HEK-293 cells transfected with the histamine H1 receptor was used as a sensitive biosensor assay for histamine release from isolated mouse mesenteric arteries. Activation of the H1 receptor by histamine was measured as an increased number of intracellular Ca(2+) transient peaks using fluorescence imaging RESULTS: The developed biosensor was sensitive to histamine in physiological relevant concentrations and responded to substances released by the artery preparation. Aldosterone treatment of mesenteric arteries from wild type mice for 50 minutes resulted in an increased number of intracellular Ca(2+) transient peaks in the biosensor cells, which was significantly inhibited by the histamine H1 blocker pyrilamine. Mesenteric arteries from mast cell deficient SASH mice induced similar pyrilamine-sensitive Ca(2+) transient response in the biosensor cells. Mesenteric arteries from wild type and SASH mice expressed histamine decarboxylase mRNA, indicating that mast cells are not the only source of histamine release.CONCLUSION: The developed biosensor assay can measure release of substances from vascular preparations. Histamine is released from the vessel preparation in response to aldosterone treatment independently of mast cells. The assay enables us to study a new signaling mechanism for vascular responses induced by aldosterone. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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