Synthesis of novel europium-labeled estradiol derivatives for time-resolved fluoroimmunoassays
| Autor: | Piitu Jauria, Petri Saviranta, Niko J. Meltola, Heikki Mikola |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Magnetic Resonance Spectroscopy
Time Factors medicine.drug_class Fluoroimmunoassay Biomedical Engineering Serum albumin Pharmaceutical Science chemistry.chemical_element Bioengineering Conjugated system Monoclonal antibody chemistry.chemical_compound Europium medicine Chelation Pharmacology Chromatography biology Estradiol Organic Chemistry Antibodies Monoclonal Standard curve chemistry biology.protein Antibody hormones hormone substitutes and hormone antagonists Derivative (chemistry) Biotechnology |
| Zdroj: | Bioconjugate chemistry. 10(3) |
| ISSN: | 1043-1802 |
| Popis: | The O-(5-carboxypentyl)-, O-(4-aminobutyl)-, O-(6-aminohexyl)oximes of 2- and 4-formylestradiol as well as the 4-carboxyethylthioether derivative of estradiol were synthesized. These estradiol derivatives were characterized using IR-, 1H-, and 13C NMR spectroscopy. All synthesized estradiol derivatives were labeled with europium chelates. These “tracers” were purified and tested in a competitive time-resolved fluoroimmunoassay with monoclonal antibody (SSI 57-2) raised against the 6-O-(carboxymethyl)oxime-bovine serum albumin derivative of estradiol. All the tested europium-labeled estradiol-4-derivatives were bound by the antibody, whereas tracers linked via position 2 were not recognized by this antibody. It was observed that tracers conjugated via C-4 gave more sensitive standard curves than tracers conjugated via C-6. Especially, the estradiol-4-thioether derivative was found to be highly useful in time-resolved fluoroimmunoassays of estradiol while using this antibody. |
| Databáze: | OpenAIRE |
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