Microbial Signature in Adipose Tissue of Crohn's Disease Patients
| Autor: | Marc Martí, Eloy Espin, Margarida Terrón-Puig, Aleidis Caro, Catalina Núñez-Roa, Joan Vendrell, Elsa Maymó-Masip, Maribel Queipo-Ortuño, M. Mar Rodríguez, Diandra Monfort-Ferré, Sonia Fernández-Veledo, Monica Millan, Carolina Serena, Lidia Sánchez-Alcoholado, Francisco J. Tinahones, Michelle Bautista, Margarita Menacho, Beatriz Espina |
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| Přispěvatelé: | [Serena,C, Monfort-Ferré,D, Terrón-Puig,M, Núñez-Roa,C, Maymó-Masip,E, Rodriguez,MM, Fernández-Veledo,S, Vendrell,J] Hospital Universitari de Tarragona Joan XXIII, Institut d’Investigació Sanitària Pere Virgili Universitat Rovira i Virgili, Tarragona, Spain. [Serena,C, Vendrell,J] CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, Madrid, Spain. [Queipo-Ortuño,M, Sanchez-Alcoholado,L] CIBER de Obesidad y Nutrición (CIBERObN), Instituto de Salud Carlos III, Madrid, Spain. [Queipo-Ortuño,M, Sanchez-Alcoholado,L] Unidad de Gestión Clínica Intercentros de Oncología Medica, Hospitales Universitarios Regional y Virgen de la Victoria de Málaga, Instituto de Investigación Biomédica de Málaga (IBIMA)-CIMES-UMA, Málaga, Spain. [Millan,M, Caro,A, Espina,B] Colorectal Surgery Unit, Hospital Universitari Joan XXIII, Tarragona, Spain. [Millan,M] Colorectal Surgery Unit, Hospital Universitari La Fe, Valencia, Spain. [Menacho,M, Bautista,M] Digestive Unit, University Hospital Joan XXIII, IISPV, Tarragona, Spain. [Tinahones,FJ] Endocrinology and Nutrition Department, Biomedical Research Institute from Malaga (IBIMA), University Hospital Virgen de la Victoria of Malaga, Malaga University, Málaga, Spain. [Espin,E, Martí,M] Colorectal Surgery Unit, General Surgery Service, Valle Hebron Hospital, Autonomous University of Barcelona, Barcelona, Spain. [Vendrell,J] Medicine and Surgery Department, School of Medicine, Universitat Rovira i Virgili, Tarragona, Spain., This study was supported by grants from the Spanish Ministry of Economy and Competitiveness (PI18/00037, PI15/00143 to C.S., PI14/00228 and PI17/01503 to J.V., and PI15/00256 to M.Q.-O., RTI2018-093919 to S.F.-V.), co-financed by the European Regional Development Fund (ERDF) and a European Crohn’s and Colitis Organization grant to C.S. The Spanish Biomedical Research Center in Diabetes and Associated Metabolic Disorders (CIBERDEM) (CB07708/0012) is an initiative of the Instituto de Salud Carlos III. C.S. acknowledges support from 'Ramón y Cajal' program from the Ministerio de Educación y Ciencia (RYC2013-13186), co-financed by the ERDF. S.F.-V. and M.Q.-O. acknowledge support from the 'Miguel Servet' tenure track program (CP10/00438, CPII16/0008, and CPI13/00003, respectively) from the Fondo de Investigación Sanitaria, co-financed by the ERDF.M.Q.-O. belongs to the regional 'Nicolas Monardes' research program of the Consejería de Salud (C-0030-2018), Junta de Andalucía, Spain, and M.T.-P. is a recipient of a Río Hortega IISCIII fellowship (CM18/00029), both co-financed by the ERDF. |
| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharide biosynthesis
Phenomena and Processes::Metabolic Phenomena::Metabolism::Metabolic Networks and Pathways [Medical Subject Headings] lcsh:Medicine Adipose tissue Gut flora lipopolysaccharide biosynthesis Chemicals and Drugs::Nucleic Acids Nucleotides and Nucleosides::Nucleic Acids::DNA::DNA Bacterial [Medical Subject Headings] medicine.disease_cause Inflammatory bowel disease tissue microbiota Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] 16S sequencing 0302 clinical medicine Diseases::Pathological Conditions Signs and Symptoms::Pathologic Processes::Hyperplasia [Medical Subject Headings] 0303 health sciences Crohn's disease biology Microbiota General Medicine Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Albumins::C-Reactive Protein [Medical Subject Headings] Organisms::Bacteria::Proteobacteria [Medical Subject Headings] Fusobacterium Anatomy::Tissues::Connective Tissue::Adipose Tissue [Medical Subject Headings] Tissue microbiota Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Data Collection::Health Surveys::Health Status Indicators::Patient Acuity::Severity of Illness Index [Medical Subject Headings] 030211 gastroenterology & hepatology Diseases::Pathological Conditions Signs and Symptoms::Pathologic Processes::Dysbiosis [Medical Subject Headings] Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Nerve Tissue Proteins::S100 Proteins::Leukocyte L1 Antigen Complex [Medical Subject Headings] Tejido adiposo Article 03 medical and health sciences inflammatory bowel disease Lipopolisacáridos medicine Escherichia coli Microbiome Enfermedad de Crohn 030304 developmental biology business.industry lcsh:R Chemicals and Drugs::Lipids::Lipopolysaccharides [Medical Subject Headings] Creeping fat Pathogenic bacteria medicine.disease biology.organism_classification digestive system diseases ARN ribosómico 16S PICRUSt analysis Tejidos Enfermedad inflamatoria intestinal Immunology creeping fat Calprotectin business Dysbiosis Diseases::Pathological Conditions Signs and Symptoms::Pathologic Processes::Inflammation [Medical Subject Headings] |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Journal of clinical medicine r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname Journal of Clinical Medicine Volume 9 Issue 8 Journal of Clinical Medicine, Vol 9, Iss 2448, p 2448 (2020) |
| ISSN: | 2077-0383 |
| Popis: | Crohn&rsquo s disease (CD) is characterized by compromised immune tolerance to the intestinal commensal microbiota, intestinal barrier inflammation, and hyperplasia of creeping fat (CF) and mesenteric adipose tissue (AT), which seems to be directly related to disease activity. Gut microbiota dysbiosis might be a determining factor in CD etiology, manifesting as a low microbial diversity and a high abundance of potentially pathogenic bacteria. We tested the hypothesis that CF is a reservoir of bacteria through 16S-rRNA sequencing of several AT depots of patients with active and inactive disease and controls. We found a microbiome signature within CF and mesenteric AT from patients, but not in subcutaneous fat. We failed to detect bacterial DNA in any fat depot of controls. Proteobacteria was the most abundant phylum in both CF and mesenteric AT, and positively correlated with fecal calprotectin/C-reactive protein. Notably, the clinical status of patients seemed to be related to the microbiome signature, as those with the inactive disease showed a reduction in the abundance of pathogenic bacteria. Predictive functional profiling revealed many metabolic pathways including lipopolysaccharide biosynthesis and sulfur metabolism overrepresented in active CD relative to that in inactive CD. Our findings demonstrate that microbiota dysbiosis associated with CD pathophysiology is reflected in AT and might contribute to disease severity. |
| Databáze: | OpenAIRE |
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