Kinetics of chemotaxis, cytokine, and chemokine release of NR8383 macrophages after exposure to inflammatory and inert granular insoluble particles
| Autor: | Isabell Schremmer, Kateryna Loza, Alexander Brik, Matthias Epple, Daniel G. Weber, Georg Johnen, Nina Rosenkranz, Alexander Rostek, T. Brüning, Jürgen Bünger, Götz A. Westphal |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chemokine medicine.medical_treatment Chemie CCL3 Inflammation CCL4 Toxicology Cell Line 03 medical and health sciences Macrophages Alveolar medicine Animals Cell Migration Assays Macrophage Titanium Air Pollutants biology Chemistry 030111 toxicology Chemotaxis Gene Expression Profiling General Medicine Quartz respiratory system Silicon Dioxide Rats CXCL1 Kinetics 030104 developmental biology Cytokine Biochemistry Biophysics biology.protein Cytokines Nanoparticles Tumor necrosis factor alpha Particulate Matter medicine.symptom Barium Sulfate Chemokines |
| Zdroj: | Toxicology letters. 263 |
| ISSN: | 1879-3169 |
| Popis: | Accumulation of macrophages and neutrophil granulocytes in the lung are key events in the inflammatory response to inhaled particles. The present study aims at the time course of chemotaxis in vitro in response to the challenge of various biopersistent particles and its functional relation to the transcription of inflammatory mediators. NR8383 rat alveolar macrophages were challenged with particles of coarse quartz, barium sulfate, and nanosized silica for one, four, and 16h and with coarse and nanosized titanium dioxide particles (rutile and anatase) for 16h only. The cell supernatants were used to investigate the chemotaxis of unexposed NR8383 macrophages. The transcription of inflammatory mediators in cells exposed to quartz, silica, and barium sulfate was analyzed by quantitative real-time PCR. Challenge with quartz, silica, and rutile particles induced significant chemotaxis of unexposed NR8383 macrophages. Chemotaxis caused by quartz and silica was accompanied by an elevated transcription of CCL3, CCL4, CXCL1, CXCL3, and TNFα. Quartz exposure showed an earlier onset of both effects compared to the nanosized silica. The strength of this response roughly paralleled the cytotoxic effects. Barium sulfate and anatase did not induce chemotaxis and barium sulfate as well caused no elevated transcription. In conclusion, NR8383 macrophages respond to the challenge with inflammatory particles with the release of chemotactic compounds that act on unexposed macrophages. The kinetics of the response differs between the various particles. |
| Databáze: | OpenAIRE |
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