Cloning and characterization of the cysAMK region of Salmonella typhimurium
| Autor: | M D Hulanicka, G Jagura-Burdzy, C Garrett, N M Kredich |
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| Předmět: |
Salmonella typhimurium
Lyases Molecular cloning Microbiology chemistry.chemical_compound Restriction map Cysteine Cloning Molecular Promoter Regions Genetic Molecular Biology Gene Genetics Cloning Cysteine Synthase biology Chromosome Mapping Chromosomes Bacterial Lambda phage biology.organism_classification Molecular biology PBR322 Restriction site chemistry Genes Bacterial DNA Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | A total of 30 kilobases of DNA comprising the cysAMK region of S. typhimurium was cloned as a series of fragments in phage lambda 1059. The genetic organization of this region was established through studies of gene expression from fragments subcloned in pBR322 and from blot hydridization analyses of restriction sites in chromosomal DNA from multisite deletion strains. The results give a gene order of cysA-cysM-crr-ptsl-ptsH-cysK over a distance of approximately 12 kilobases. cysM and cysA have been cloned and expressed in pBR322; attempts to obtain stable pBR322 derivatives carrying cysK were unsuccessful. |
| Databáze: | OpenAIRE |
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