Monitoring of CD38high expression in peripheral blood CD8+ lymphocytes in patients after kidney transplantation as a marker of cytomegalovirus infection
| Autor: | Milan Kuman, Pavel Studenik, Jiri Litzman, Olga Ticha, Martina Stouracova, Tomáš Freiberger |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Graft Rejection Male Lymphocyte medicine.medical_treatment Immunology Congenital cytomegalovirus infection Cytomegalovirus 030230 surgery CD38 CD8-Positive T-Lymphocytes Sensitivity and Specificity 03 medical and health sciences 0302 clinical medicine Predictive Value of Tests medicine Immunology and Allergy Humans Kidney transplantation 030304 developmental biology Monitoring Physiologic Retrospective Studies 0303 health sciences Transplantation business.industry virus diseases Immunosuppression Middle Aged medicine.disease ADP-ribosyl Cyclase 1 Kidney Transplantation 3. Good health medicine.anatomical_structure Predictive value of tests Cytomegalovirus Infections business Complication Biomarkers |
| Zdroj: | Transplant immunology. 24(1) |
| ISSN: | 1878-5492 |
| Popis: | Background Cytomegalovirus (CMV) infection is a life-threatening complication after solid organ transplantation. It usually appears in the first months after transplantation as a consequence of immunosuppression. The goal of this study was to evaluate the clinical significance of CD38high/CD3+8+ percentages in the detection of CMV infection in patients after kidney transplantation. Methods In this retrospective study, 269 patients were monitored 2–3 months after renal transplantation for the percentage of CD38high/CD3+8+ lymphocytes estimated by flow cytometry and for the number of CMV DNA copies in peripheral blood using a real-time polymerase chain reaction. Results CMV infection was diagnosed in 12 (4.5%) patients between the 31st and 63rd days after transplantation, and all of them had percentages of CD38high/CD3+8+ T lymphocytes above 20%. In 4 of them, CMV DNAemia in peripheral blood was not detected, and 2 of these suffered from tissue-invasive CMV disease. In 7 patients with CMV DNAemia, the CD38high/CD3+8+ T lymphocyte percentage did not exceed 20%, and these patients did not develop CMV infection requiring antiviral treatment. In 23 additional patients, a CD38high/CD3+CD8+ percentage above 20% was recorded without CMV DNAemia. All of the remaining 234 patients never exceeded the arbitrary limit of 20%. The estimated sensitivity and specificity were 100% and 91% using clinical decision on the presence of CMV infection as a reference value, respectively. The estimated negative predictive value was 100%; however, the estimated positive predictive value was quite low (34%). Conclusions The CD38high/CD3+8+ lymphocyte percentage seems to be a useful additional diagnostic marker for CMV infection in patients after kidney transplantation, especially when patients are in the risk of a tissue-invasive disease when CMV DNA copies may not be detectable in peripheral blood. |
| Databáze: | OpenAIRE |
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