Guided folding takes a start from the molecular imprinting of structured epitopes
| Autor: | Graziano Guella, Erika Andreetto, Alessandra Bossi, Andrea Anesi, Emmanuele Ambrosi, Lucia Cenci |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
inorganic chemicals
0301 basic medicine chemistry.chemical_classification Materials science molecularly imprinted nanoparticles catalysis of cystine bonds technology industry and agriculture Nanotechnology Peptide folding assistance Polymeric nanoparticles Epitope Folding (chemistry) 03 medical and health sciences 030104 developmental biology Molecular recognition chemistry structured peptide General Materials Science Biomimetics Molecular imprinting structured epitope recognition |
| Zdroj: | Nanoscale. 8:15665-15670 |
| ISSN: | 2040-3372 2040-3364 |
| Popis: | A biomimetic route towards assisted folding was explored. Molecularly imprinted polymeric nanoparticles (MIP NPs), i.e. biomimetics with entailed molecular recognition properties made by a template assisted synthesis, were prepared to target a structured epitope: the cystine containing peptide CC9ox, which corresponds to the apical portion of the β-hairpin hormone Hepcidin-25. The structural selection was achieved by the MIP NPs; moreover, the MIP NPs demonstrated favouring the folding of the linear random peptide (CC9red) into the structured one (CC9ox), anticipating the future role of the MIP NPs as in situ nanomachines to counteract folding defects. |
| Databáze: | OpenAIRE |
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