Characterization of α-D-glucan produced by a probiont Enterococcus hirae KX577639 from feces of south Indian Irula tribals
| Autor: | Palanisamy Bruntha Devi, Digambar Kavitake, Venkatesan Brindha Priyadarisini, Prathapkumar Halady Shetty, Suresh Rajendran, Jabastin Jayamanohar |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Magnetic Resonance Spectroscopy Sucrose Starch 02 engineering and technology Microscopy Atomic Force 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry law.invention Homopolysaccharide Feces chemistry.chemical_compound Probiotic X-Ray Diffraction Structural Biology law Enterococcus hirae 010608 biotechnology Spectroscopy Fourier Transform Infrared Humans Food science Glucans Molecular Biology Glucan chemistry.chemical_classification Strain (chemistry) biology Probiotics General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Gastrointestinal Microbiome Monomer Solubility chemistry 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 118:1667-1675 |
| ISSN: | 0141-8130 |
| Popis: | Exopolysaccharide (EPS) producing probiotic strain Enterococcus hirae KX577639 were isolated from the feces of South Indian Irula tribes. EPS yield was 18.57 g/L (dry weight) at 48 h in 2% sucrose supplemented MRS medium. TLC and GC–MS analysis confirmed the presence of predominant glucose monomer indicating the homopolysaccharide nature of EPS. FTIR and NMR studies revealed that the EPS were branched α-D-glucan polymer with α-(1 → 6) and α-(1 → 3) linkages. SEM analysis of glucan-EPS revealed porous and starch like cracked granules of aggregation. AFM studies proved spherical lumps and dense, grainy like network. The thermal behavior of glucan-EPS showed degradation temperature of 315.98 °C and melting point of 296.67 °C. The XRD analysis confirmed the amorphous nature of EPS with a crystalline index of 0.48. The water solubility index and water holding capacity of glucan-EPS showed 46.5% and 202.04%. These distinctive features of the glucan EPS could find its potential application in functional food products as the α-(1 → 3) linkage are resistant to human digestive enzymes and can serve as a nutrient to gut bacteria. This the first study reporting the EPS production by Enterococcus hirae. |
| Databáze: | OpenAIRE |
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