Krüppel-like transcription factor 8 (Klf8) is expressed and active in the neurons of the mouse brain
| Autor: | Nikola Habek, Katarina Kapuralin, Marija Ćurlin, Srećko Gajović, Marina Dobrivojević |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cerebellum
Krüppel-like factor 8 Mouse brain Neurons Transcription factor Kruppel-Like Transcription Factors Hippocampus Gene Expression Mice Transgenic Striatum Biology 03 medical and health sciences 0302 clinical medicine medicine Genetics Animals 030304 developmental biology Cell Nucleus 0303 health sciences Brain General Medicine Molecular biology Olfactory bulb Mice Inbred C57BL medicine.anatomical_structure Cerebral cortex Organ Specificity Brain Gray Matter Nucleus 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Gene. 570(1) |
| ISSN: | 1879-0038 |
| Popis: | Krüppel-like transcription factor 8 (KLF8) is a transcription factor suggested to be involved in various cellular events, including malignant cell transformation, still its expression in the adult rodent brain remained unknown. To analyze Klf8 in the mouse brain and to identify cell types expressing it, a specific transgenic Klf8Gt1Gaj mouse was used. The resulting Klf8 gene-driven β-galactosidase activity was visualized by X-gal histochemical staining of the brain sections. The obtained results were complemented by in situ RNA hybridization and immunohistochemistry. Klf8 was highly expressed throughout the adult mouse brain gray matter including the cerebral cortex, hippocampus, olfactory bulb, hypothalamus, pallidum, and striatum, but not in the cerebellum. Immunofluorescent double-labeling revealed that KLF8-immunoreactive cells were neurons, and the staining was located in their nucleus. This was the first study showing that Klf8 was highly expressed in various regions of the mouse brain and in particular in the neurons, where it was localized in the cell nuclei. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect