New insight on the relationship between lethal electrical fields versus cardiomyocyte orientation
| Autor: | Pedro Xavier de Oliveira, Fernanda dos Santos Costa Leomil |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Defibrillation medicine.medical_treatment lcsh:Biotechnology Cell Biomedical Engineering Stimulation 030204 cardiovascular system & hematology Stimulus (physiology) 03 medical and health sciences 0302 clinical medicine Orientation (mental) Internal medicine lcsh:TP248.13-248.65 Medicine lcsh:R5-920 business.industry Cell electrical stimulation medicine.disease medicine.anatomical_structure Shock (circulatory) Ventricular fibrillation Cardiology medicine.symptom business lcsh:Medicine (General) Perfusion Probability of cellular lethality 030217 neurology & neurosurgery |
| Zdroj: | Research on Biomedical Engineering v.34 n.3 2018 Research on Biomedical Engineering Sociedade Brasileira de Engenharia Biomédica (SBEB) instacron:SBEB Research on Biomedical Engineering, Vol 34, Iss 3, Pp 226-233 (2018) Research on Biomedical Engineering, Volume: 34, Issue: 3, Pages: 226-233, Published: 13 SEP 2018 Research on Biomedical Engineering, Issue: ahead, Published: 13 SEP 2018 |
| Popis: | Introduction Cardiovascular diseases represent a major cause of death world-wide and one of their greatest complications is the development of cardiac arrhythmias, in which ventricular fibrillation (VF) stands out as the most severe one. The only therapy that reverses VF is defibrillation. However defibrillatory shock is capable of killing heart cells and it is known that the orientation of the cell major axis with respect to the electrical field (E) direction is a determining factor for cellular excitation and injury, which is leading to the development of new defibrillation protocols. The aim of this work is to fill the gap in information about cell lethality for intermediate cell orientation angles. Methods Ventricular myocytes were extracted from adult male Wistar rats and the cells were plated in a chamber for perfusion and stimulation with bipolar voltage pulses to determine the stimulation threshold (ET). Then, monopolar stimulus was applied and amplitude was increased until cell lethal injury. This protocol was performed on four experimental groups: cells oriented at 0°, 30°, 60° and 90°, with respect to E direction. Results 87 cells were analyzed and an increase in amplitude of E associated with 50% lethality (E50) was verified as the direction of E application and cell major axis orientation departed. Conclusion Taken the same probability of lethality, our data suggest a nonlinear increase of E amplitude from 0° to 90° similar to that of ET. These in-between data had not yet been shown and are important for service-based future defibrillation protocols. |
| Databáze: | OpenAIRE |
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