Oxidative damage to biological macromolecules in Prague bus drivers and garagemen: Impact of air pollution and genetic polymorphisms
| Autor: | Irena Chvatalova, Ivo Solansky, Vlasta Svecova, Alena Milcova, Radim J. Sram, Pavel Rossner, Zdena Lnenickova, Bozena Novotna, Yana Bagryantseva |
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| Rok vydání: | 2010 |
| Předmět: |
Male
Guanine Air Pollutants Occupational Biology Dinoprost Toxicology medicine.disease_cause DNA Glycosylases Protein Carbonylation Lipid peroxidation chemistry.chemical_compound Air Pollution Genotype medicine Humans Vasoconstrictor Agents Genetic Predisposition to Disease Lymphocytes Polycyclic Aromatic Hydrocarbons Carcinogen Glutathione Transferase Vehicle Emissions Xeroderma Pigmentosum Group D Protein chemistry.chemical_classification Volatile Organic Compounds Polymorphism Genetic DNA General Medicine Comet assay Oxidative Stress Enzyme chemistry Biochemistry 8-Hydroxy-2'-Deoxyguanosine Comet Assay Lipid Peroxidation Oxidative stress DNA Damage Nucleotide excision repair |
| Zdroj: | Toxicology Letters. 199:60-68 |
| ISSN: | 0378-4274 |
| DOI: | 10.1016/j.toxlet.2010.08.007 |
| Popis: | DNA integrity was investigated in the lymphocytes of 50 bus drivers, 20 garagemen and 50 controls using the comet assay with excision repair enzymes. In parallel, 8-oxo-7,8-dihydro-2′-deoxyguanosine and 15-F2t-isoprostane levels in the urine and protein carbonyl levels in the plasma were assessed as markers of oxidative damage to DNA, lipids and proteins. Exposure to carcinogenic polycyclic aromatic hydrocarbons (cPAHs) and volatile compounds was measured by personal samplers for 48 and 24 h, respectively, before the collection of biological specimens. Both exposed groups exhibited a higher levels of DNA instability and oxidative damage to biological macromolecules than the controls. The incidence of oxidized lesions in lymphocyte DNA, but not the urinary levels of 8-oxodG, correlated with exposure to benzene and triglycerides increased this damage. Oxidative damage to lipids and proteins was associated with exposure to cPAHs and the lipid peroxidation levels positively correlated with age and LDL cholesterol, and negatively with vitamin C. The carriers of at least one variant hOGG1 (Cys) allele tended to higher oxidative damage to lymphocyte DNA than those with the wild genotype, while XPD23 (Gln/Gln) homozygotes were more susceptible to the induction of DNA strand breaks. In contrast, GSTM1 null variant seemed to protect DNA integrity. |
| Databáze: | OpenAIRE |
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