Differential regulation of protein kinase C isoforms in human uterine leiomyoma
| Autor: | M. Breuiller-Fouché, M.C. Vacher-Lavenu, C. Tertrin-Clary, Charles Chapron, Françoise Ferré, Isabelle Eude |
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| Rok vydání: | 2001 |
| Předmět: |
Gene isoform
Adult medicine.medical_specialty Blotting Western Immunoblotting Biology Isozyme MAP2K7 Internal medicine medicine Humans Protein kinase C Phorbol 12 13-Dibutyrate Protein Kinase C Uterine leiomyoma Endothelin-1 Leiomyoma Uterus Myometrium Obstetrics and Gynecology Middle Aged musculoskeletal system medicine.disease Molecular biology Endothelin 1 female genital diseases and pregnancy complications Isoenzymes Endocrinology Reproductive Medicine Uterine Neoplasms Female Subcellular Fractions |
| Zdroj: | Gynecologic and obstetric investigation. 51(3) |
| ISSN: | 0378-7346 |
| Popis: | The protein kinase C (PKC) isoenzyme expression pattern in human uterine leiomyoma was compared with that obtained in homologous myometrium distal from the tumor. The six PKC isoforms (PKCα, PKCβ1, PKCβ2, PKCδ, PKCΕ and PKCζ) evidenced in the myometrium were found to be similarly expressed in leiomyoma. Quantitative immunoblotting revealed that all PKC isoforms were preferentially localized in the particulate fraction. To gain insight into the possible functional consequences of PKC expression patterns, subcellular redistribution in response to the mitogenic peptide endothelin-1 (ET-1) was studied. After stimulation with ET-1, differential redistribution occurred in leiomyoma and myometrium, suggesting a selective role of PKC isoforms in the myometrial growth process. |
| Databáze: | OpenAIRE |
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