CD52 is a novel costimulatory molecule for induction of CD4+ regulatory T cells
| Autor: | Seiji Minota, Yasunori Yamaguchi, Shuji Kaga, Yasunori Uemura, Toshiyuki Tanaka, Yoshiaki Sohma, Tetsuto Kobayashi, Kaori Horie, Hiroko Inazawa, Jun-Ichi Masuyama, Kumiko Kojima, Yumi Aoki, Isao Serizawa, Tomoko Watanabe |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Interleukin 2
CD52 Antibodies Neoplasm Immunology Epitopes T-Lymphocyte Graft vs Host Disease chemical and pharmacologic phenomena Mice SCID CD8-Positive T-Lymphocytes Antibodies Monoclonal Humanized Lymphocyte Activation T-Lymphocytes Regulatory Enterotoxins Mice Interleukin 21 Antigen Antigens CD Antigens Neoplasm medicine Animals Humans Immunology and Allergy Alemtuzumab Glycoproteins CD40 biology Reverse Transcriptase Polymerase Chain Reaction Antibodies Monoclonal Forkhead Transcription Factors hemic and immune systems T lymphocyte Molecular biology CD52 Antigen Polyclonal B cell response biology.protein Interleukin-2 Female CD8 medicine.drug |
| Zdroj: | Clinical Immunology. 120:247-259 |
| ISSN: | 1521-6616 |
| DOI: | 10.1016/j.clim.2006.05.006 |
| Popis: | We previously reported that 4C8 monoclonal antibody (mAb) provides a costimulatory signal to human CD4+ T cells and consequently induces regulatory T (Treg) cells, which are hypo-responsive and suppress the polyclonal response of bystander CD4+ cells in a contact-dependent manner. In this study, we identified the antigen of 4C8 mAb as CD52. Costimulation with Campath-1H, a humanized anti-CD52 mAb, also induced Treg cells. Anti-CD52-induced Treg cells suppressed the proliferation of both CD4+ and CD8+ T cells provided with polyclonal or allogeneic stimulation. When Treg cells were induced from Staphylococcal enterotoxin B (SEB) treated cells, they suppressed the response to SEB more efficiently than that to another superantigen, SEA. Furthermore, anti-CD52-induced Treg cells could be expanded by culture with IL-2 followed by CD52-costimulation, and co-injection of expanded Treg cells suppressed lethal xenogeneic graft versus host disease (GvHD) reactions in SCID mice caused by human peripheral blood mononuclear cells (PBMCs). |
| Databáze: | OpenAIRE |
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