Regulation of FUSCA3 Expression During Seed Development in Arabidopsis
| Autor: | Caroline Michaud, Thomas J. Roscoe, Bertrand Dubreucq, Loïc Lepiniec, Fernando Clavijo, Dao-Xiu Zhou, Zsolt Kelemen, Gaël Paszkiewicz, Martine Devic, Virginie Vaissayre |
|---|---|
| Přispěvatelé: | Diversité, adaptation, développement des plantes (UMR DIADE), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), University of Tübingen, Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Océanographie Microbienne (LOMIC), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), the French NationalResearch Agency [ANR, grant CERES (ANR-10-BLAN-123801)and ANR-BLAN-10-123801 to V.V., F.C. and G.P.]. The InstitutJean-Pierre Bourgin benefits from the support of the LabexSaclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS], ANR-10-BLAN-1238,CERES,Contrôle de l'expression des gènes du REseau de régulation 'AFL' et développement de la graine(2010), ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Banyuls (OOB), Institut de Recherche en Horticulture et Semences (IRHS), AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)-Université d'Angers (UA), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis thaliana Physiology seed maturation Arabidopsis Plant Science qrabidopsis thaliana 01 natural sciences fegulation of expression 03 medical and health sciences Transcription (biology) Gene Expression Regulation Plant Two-Hybrid System Techniques Seed maturation Epigenetics Seed reserves seed reserves Promoter Regions Genetic Psychological repression ComputingMilieux_MISCELLANEOUS biology Arabidopsis Proteins FUSCA3 Regulation of expression [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Cell Biology General Medicine biology.organism_classification WRKY protein domain Cell biology [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding 030104 developmental biology Regulatory sequence Fus3 Seeds biology.protein PRC2 WRKY2 010606 plant biology & botany Transcription Factors |
| Zdroj: | Plant and Cell Physiology Plant and Cell Physiology, Oxford University Press (OUP), 2019, 60 (2), pp.476-487. ⟨10.1093/pcp/pcy224⟩ Plant and Cell Physiology, 2019, 60 (2), pp.476-487. ⟨10.1093/pcp/pcy224⟩ |
| ISSN: | 0032-0781 1471-9053 |
| DOI: | 10.1093/pcp/pcy224⟩ |
| Popis: | International audience; FUSCA3 (FUS3) is a master regulator of seed development important in establishing and maintaining embryonic identity whose expression is tightly regulated at genetic and epigenetic levels. Despite this prominent role, the control of FUS3 expression remains poorly understood. Promoter and functional complementation analyses provided insight into the regulation of FUS3. W-boxes present in the promoter proximal to the start of transcription are recognized by WRKY type-1 factors which are necessary for the activation of FUS3 expression. The RY motif, the binding site of B3 factors, is important for the activation of FUS3 in the embryo proper but not in the suspensor. The loss of a negative regulatory sequence (NRS) leads to preferential expression of FUS3 in the vasculature of vegetative tissues. Since the NRS includes the RY motif, mechanisms of activation and repression target adjacent or overlapping regions. These findings discriminate the regulation of FUS3 from that of LEAFY COTYLEDON2 by the control exerted by WRKY factors and by the presence of the RY motif, yet also confirm conservation of certain regulatory elements, thereby implicating potential regulation by BASIC PENTACYSTEINE (BPC) factors and POLYCOMB REPRESSIVE COMPLEX2 (PRC2). |
| Databáze: | OpenAIRE |
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