Evidence that endogenous vasoactive intestinal peptide (VIP) plays a role in the maintenance of the growth and steroidogenic capacity of rat adrenal zona glomerulosa
| Autor: | Cinzia Tortorella, Piera Rebuffat, Krzysztof W. Nowak, Gastone G. Nussdorfer, Francesco G. Musajo, Giuseppina Mazzocchi, Giuseppe Gottardo |
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| Rok vydání: | 1994 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.drug_class Endocrinology Diabetes and Metabolism Clinical Biochemistry Vasoactive intestinal peptide Blood Pressure Biology Biochemistry Plasma renin activity chemistry.chemical_compound Endocrinology Adrenocorticotropic Hormone Corticosterone Internal medicine Renin medicine Animals Rats Wistar Molecular Biology Aldosterone Adrenal cortex Angiotensin II Cell Biology Hypertrophy Mitochondria Rats medicine.anatomical_structure chemistry Zona glomerulosa Molecular Medicine Corticosteroid Zona Glomerulosa Adrenal medulla hormones hormone substitutes and hormone antagonists Vasoactive Intestinal Peptide |
| Zdroj: | The Journal of steroid biochemistry and molecular biology. 51(1-2) |
| ISSN: | 0960-0760 |
| Popis: | The effects of a 7-day intraperitoneal infusion with VIP (0.03 nmol.kg-1.min-1) and its antagonist [4-Cl-D-Phe6,Leu17]-VIP (VIP-A; 3 nmol.kg-1.min-1) were studied in sham and bilaterally adrenalectomized rats bearing ACTH and angiotensin II (ANG-II)-responsive adrenocortical autotransplants. VIP significantly increased plasma aldosterone (ALDO) concentration (PAC) and lowered plasma renin activity (PRA) in both groups of animals, without affecting plasma levels of ACTH and corticosterone. This treatment caused a marked hypertrophy of adrenal zona glomerulosa (ZG) and its parenchymal cells (without inducing any significant change in the zona-fasciculata morphology), as well as of ZG-like cells of autotransplants. Isolated ZG cells and autotransplant quarters obtained from VIP-infused rats evidenced a notable increase in both their basal and maximally ACTH- or ANG-II-stimulated ALDO secretion. The simultaneous infusion of rats with VIP-A completely reversed all these effects of VIP. The infusion with VIP-A alone caused, in sham-operated rats, a net decrease in PAC, coupled with a rise in PRA, and a marked atrophy of ZG and ZG cells; basal and maximally stimulated ALDO secretion of dispersed ZG cells was also significantly lowered. Conversely, VIP-A did not evoke any appreciable effect in autotransplanted rats. These findings suggest that endogenous VIP is specifically involved in the maintenance of the growth and secretory capacity of rat adrenal ZG. Since regenerated adrenocortical autotransplants, which are responsive to VIP but not to VIP-A infusion, are completely deprived of chromaffin cells, the hypothesis is advanced that adrenal medulla may be the source of endogenous VIP regulating ZG function. |
| Databáze: | OpenAIRE |
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