| Autor: |
David Skufca, Darja Bozic, Matej Hocevar, Marko Jeran, Apolonija Bedina Zavec, Matic Kisovec, Marjetka Podobnik, Rok Tomazin, Tadeja Matos, Ales Iglic, Tjasa Griessler Bulc, Ester Heath, Veronika Kralj-Iglic |
| Rok vydání: |
2022 |
| Předmět: |
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| DOI: |
10.20944/preprints202206.0244.v1 |
| Popis: |
Small cellular particles are released into the surroundings of cells and are proposed to play an important role in intercellular communication and consequently the responses of microbial com-munities to environmental stressors. We studied the connection between the small cellular parti-cles and the efficiency of three culture series of the microalge Phaeodactylum tricornutum and bac-teria (axenic microalgae, bacterial culture and co-culture of the two) in removing bisphenols from their growth medium. The microorganism growth rate was determined by flow cytometry, protein profiles were examined by protein gel electrophoresis, cultures and small cellular particle isolates were imaged by scanning electron microscopy, and bisphenols were analyzed using gas chroma-tography coupled with tandem mass spectrometry (GC-MS/MS). Higher growth rates of microal-gae were observed in the co-culture than in the axenic microalgal culture, while the presence of bisphenols neither influenced the morphology of the microalgal cells, protein profiles, nor the small cellular particle isolates. Biotic removal of bisphenols ranged from 0% to 71% and differed among the culture series in a compound-specific manner. However, it remains unclear which mechanisms influenced algal growth and bisphenol removal. Further research on the mechanisms of interspecies communication is needed to advance our understanding of microbial communities at the nano-level. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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