Combination treatment of neonatal rats with hypoxia-ischemia and endotoxin induces long-lasting memory and learning impairment that is associated with extended cerebral damage
Autor: | Tomoaki Ikeda, Kenichi Mishima, Tsuyomu Ikenoue, Naoya Aoo, Nobuaki Egashira, Michihiro Fujiwara, Katsunori Iwasaki |
---|---|
Rok vydání: | 2004 |
Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty medicine.medical_treatment Models Neurological Central nervous system Striatum Water maze Sensitivity and Specificity Lesion Random Allocation Reference Values Internal medicine Brain Injury Chronic Avoidance Learning Reaction Time medicine Animals Memory impairment Memory disorder Rats Wistar Maze Learning Saline Probability Analysis of Variance Memory Disorders Learning Disabilities business.industry Memoria Obstetrics and Gynecology medicine.disease Combined Modality Therapy Rats Surgery Endotoxins Disease Models Animal Endocrinology medicine.anatomical_structure Animals Newborn Hypoxia-Ischemia Brain Female medicine.symptom business |
Zdroj: | American Journal of Obstetrics and Gynecology. 191:2132-2141 |
ISSN: | 0002-9378 |
DOI: | 10.1016/j.ajog.2004.04.039 |
Popis: | Objective We assessed the long-term effects of perinatal hypoxia-ischemia and endotoxin on attention and short- and long-term memory in neonatal rats with the use of behavioral tasks and brain histologic results. Study design Four hours after injections of lipopolysaccharide (1 mg/kg, intraperitoneally) or saline solution, 7-day-old Wistar rat pups were subjected to unilateral hypoxia-ischemia for 1 hour. We studied 4 groups: controls (n = 43 rats), lipopolysaccharide alone (n = 12 rats), hypoxia-ischemia alone (n = 29 rats), and combined lipopolysaccharide + hypoxia-ischemia treatment (n = 34 rats). Seven to 16 weeks after the treatment, we measured attention with a choice reaction time task, short-term memory with an 8-arm radial maze task, and long-term memory with a water maze task. At 19 weeks of age, the brain was removed, fixed, and sectioned coronally; and the volume of each part was measured. Results A loss of volume in the hippocampus was observed in the lipopolysaccharide, hypoxia-ischemia, and lipopolysaccharide + hypoxia-ischemia groups; a loss of striatum was observed in the hypoxia-ischemia and lipopolysaccharide + hypoxia-ischemia groups, but loss of cortex was observed only in the lipopolysaccharide + hypoxia-ischemia group. The lipopolysaccharide, hypoxia-ischemia, and lipopolysaccharide + hypoxia-ischemia groups showed significantly poorer performance (attention deficit) than controls in the choice reaction time task. Correct choices decreased, and error increased in the lipopolysaccharide + hypoxia-ischemia group compared with the other groups in the radial maze task, which shows short-term memory impairment. Swimming distance was significantly greater in the hypoxia-ischemia and lipopolysaccharide + hypoxia-ischemia groups than in the other 2 groups in the water maze test, which shows long-term memory impairment. Conclusion Combined lipopolysaccharide and hypoxia-ischemia treatment synergistically induced short-term memory impairment that is associated with loss of cortical volume. |
Databáze: | OpenAIRE |
Externí odkaz: |
načítá se...