Altered developmental programs and oriented cell divisions lead to bulky bones during salamander limb regeneration
Autor: | Marketa Kaucka, Alberto Joven Araus, Marketa Tesarova, Joshua D. Currie, Johan Boström, Michaela Kavkova, Julian Petersen, Zeyu Yao, Anass Bouchnita, Andreas Hellander, Tomas Zikmund, Ahmed Elewa, Phillip T. Newton, Ji-Feng Fei, Andrei S. Chagin, Kaj Fried, Elly M. Tanaka, Jozef Kaiser, András Simon, Igor Adameyko |
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Rok vydání: | 2022 |
Předmět: |
Mammals
Multidisciplinary cartilage development Bone development Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology) Molecular Biology Microbiology Biochemistry or Biopharmacy) Urodela General Physics and Astronomy General Chemistry Bone and Bones General Biochemistry Genetics and Molecular Biology Cartilage Osteogenesis Animals Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi) molekylärbiologi mikrobiologi biokemi eller biofarmaci) Cell Division |
Zdroj: | Nature Communications |
ISSN: | 2041-1723 |
Popis: | There are major differences in duration and scale at which limb development and regeneration proceed, raising the question to what extent regeneration is a recapitulation of development. We address this by analyzing skeletal elements using a combination of micro-CT imaging, molecular profiling and clonal cell tracing. We find that, in contrast to development, regenerative skeletal growth is accomplished based entirely on cartilage expansion prior to ossification, not limiting the transversal cartilage expansion and resulting in bulkier skeletal parts. The oriented extension of salamander cartilage and bone appear similar to the development of basicranial synchondroses in mammals, as we found no evidence for cartilage stem cell niches or growth plate-like structures during neither development nor regeneration. Both regenerative and developmental ossification in salamanders start from the cortical bone and proceeds inwards, showing the diversity of schemes for the synchrony of cortical and endochondral ossification among vertebrates. Normal limb development relies on synchronized formation of cartilage and bone. Here, the authors show that in salamander limb regeneration these processes are decoupled: ossification occurs after the final size of regenerating cartilage is reached, allowing fast regeneration and leading to bulky bones. eSSENCE - An eScience Collaboration |
Databáze: | OpenAIRE |
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