Effect of Embryo Vitrification on the Steroid Biosynthesis of Liver Tissue in Rabbit Offspring

Autor: Marta Domínguez-Martínez, Jose S. Vicente, Gianfranco Diretto, David S. Peñaranda, María Pilar Viudes-de-Castro, Ximo Garcia-Dominguez, Francisco Marco-Jiménez
Přispěvatelé: Marco-jimenez, F., Garcia-dominguez, X., Dominguez-martinez, M., Viudes-de-castro, M. P., Diretto, G., Penaranda, D. S., Vicente, J. S.
Rok vydání: 2020
Předmět:
0301 basic medicine
Reproductive technology
PRODUCCION ANIMAL
Cryopreservation
lcsh:Chemistry
stress
0302 clinical medicine
lcsh:QH301-705.5
Spectroscopy
IGF‐I
030219 obstetrics & reproductive medicine
Embryo
General Medicine
steroid biosynthesis
Embryo transfer
Computer Science Applications
IGF-I
Cholesterol
BIOLOGIA ANIMAL
Liver
Metabolome
Female
metabolome
Assisted reproductive technologies
Rabbits
RT‐qPCR
Steroid biosynthesis
Offspring
embryo
Biology
Stress
Catalysis
Article
Inorganic Chemistry
Andrology
03 medical and health sciences
Animals
Physical and Theoretical Chemistry
Molecular Biology
assisted reproductive technologies
Organic Chemistry
RT-qPCR
cholesterol
Embryo Transfer
Embryo
Mammalian
Embryonic stem cell
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Liver function
Zdroj: International Journal of Molecular Sciences
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname
Volume 21
Issue 22
International Journal of Molecular Sciences, Vol 21, Iss 8642, p 8642 (2020)
ISSN: 1422-0067
Popis: [EN] Preimplantation embryo manipulations during standard assisted reproductive technologies (ART) have significant repercussions on offspring. However, few studies to date have investigated the potential long-term outcomes associated with the vitrification procedure. Here, we performed an experiment to unravel the particular effects related to stress induced by embryo transfer and vitrification techniques on offspring phenotype from the foetal period through to prepuberal age, using a rabbit model. In addition, the focus was extended to the liver function at prepuberal age. We showed that, compared to naturally conceived animals (NC), offspring derived after embryo exposure to the transfer procedure (FT) or cryopreservation-transfer procedure (VT) exhibited variation in growth and body weight from foetal life to prepuberal age. Strikingly, we found a nonlinear relationship between FT and VT stressors, most of which were already present in the FT animals. Furthermore, we displayed evidence of variation in liver function at prepuberal age, most of which occurred in both FT and VT animals. The present major novel finding includes a significant alteration of the steroid biosynthesis profile. In summary, here we provide that embryonic manipulation during the vitrification process is linked with embryo phenotypic adaptation detected from foetal life to prepuberal age and suggests that this phenotypic variation may be associated, to a great extent, with the effect of embryo transfer.
This research was funded by Conselleria d'Educacio, Investigacio, Cultura i Esport, Spain, grant number AICO/2019/272. Ximo Garcia-Dominguez was supported by a research grant from the Ministry of Economy, Industry and Competitiveness of Spain (BES-2015-072429).
Databáze: OpenAIRE
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