The chromatin-modifying protein HUB2 is involved in the regulation of lignin composition in xylem vessels
| Autor: | Nicolas Delhomme, Pál Miskolczi, Bernadette Sztojka, Hannele Tuominen, Maxime Chantreau, Bo Zhang, András Gorzsás, László Bakó, Sacha Escamez, Rishikesh P. Bhalerao, Carolin Seyfferth |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Ubiquitin-Protein Ligases xylem vessels Arabidopsis lignin Plant Science macromolecular substances 01 natural sciences Cell wall 03 medical and health sciences chemistry.chemical_compound Cell Wall Gene Expression Regulation Plant Xylem Lignin Arabidopsis thaliana PIRIN2 biology Arabidopsis Proteins AcademicSubjects/SCI01210 fungi Botany food and beverages Botanik biology.organism_classification Research Papers Chromatin 030104 developmental biology HUB2 chemistry cell wall chemistry Biophysics Growth and Development syringyl-type lignin Secondary cell wall Chemical fingerprinting 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany |
| Popis: | PIRIN2 (PRN2) was earlier reported to suppress syringyl (S)-type lignin accumulation of xylem vessels of Arabidopsis thaliana. In the present study, we report yeast two-hybrid results supporting the interaction of PRN2 with HISTONE MONOUBIQUITINATION2 (HUB2) in Arabidopsis. HUB2 has been previously implicated in several plant developmental processes, but not in lignification. Interaction between PRN2 and HUB2 was verified by β-galactosidase enzymatic and co-immunoprecipitation assays. HUB2 promoted the deposition of S-type lignin in the secondary cell walls of both stem and hypocotyl tissues, as analysed by pyrolysis-GC/MS. Chemical fingerprinting of individual xylem vessel cell walls by Raman and Fourier transform infrared microspectroscopy supported the function of HUB2 in lignin deposition. These results, together with a genetic analysis of the hub2 prn2 double mutant, support the antagonistic function of PRN2 and HUB2 in deposition of S-type lignin. Transcriptome analyses indicated the opposite regulation of the S-type lignin biosynthetic gene FERULATE-5-HYDROXYLASE1 by PRN2 and HUB2 as the underlying mechanism. PRN2 and HUB2 promoter activities co-localized in cells neighbouring the xylem vessel elements, suggesting that the S-type lignin-promoting function of HUB2 is antagonized by PRN2 for the benefit of the guaiacyl (G)-type lignin enrichment of the neighbouring xylem vessel elements. |
| Databáze: | OpenAIRE |
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