Genomic Structure and Functional Analysis of Promoter Region of Somatolactin Gene of Sea Bream (Sparus aurata)
| Autor: | Josep A. Calduch-Giner, Jaume Pérez-Sánchez, Manuela Ortiz, Antonio Astola, Manuel M. Valdivia |
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| Rok vydání: | 2004 |
| Předmět: |
Fish Proteins
Sequence analysis TATA box Immunoblotting Molecular Sequence Data CHO Cells Transfection Applied Microbiology and Biotechnology Cricetulus Cricetinae Consensus sequence Animals Luciferase Genomic library Cloning Molecular Luciferases Promoter Regions Genetic Gene Glycoproteins Genomic Library Rous sarcoma virus Base Sequence Estradiol Symporters biology Sodium-Phosphate Cotransporter Proteins Promoter Sequence Analysis DNA biology.organism_classification Molecular biology Sea Bream Pituitary Hormones Gene Components Verapamil Pituitary Gland Tetradecanoylphorbol Acetate |
| Zdroj: | Marine Biotechnology. 6:411-418 |
| ISSN: | 1436-2236 1436-2228 |
| DOI: | 10.1007/s10126-004-3210-2 |
| Popis: | Somatolactin (SL) is a pituitary hormone belonging to the growth hormone-prolactin family and is produced in the intermediate lobe of teleosts. The SL gene was isolated from a sea bream genomic library and found to be composed of 5 exons distributed within a 9-kb length of DNA. Sequence analysis of the proximal promoter region showed the presence of a classical TATA box located 59 bp upstream from the initial start ATG codon, 5 consensus sequences corresponding to the Pit-1 binding element, and a putative CREB site. In CHO cells cotransfected with the DNA from 2 plasmids, one encoding sea bream Pit-1 under Rous sarcoma virus long terminal repeat regulation and one encoding the SL promoter driving the expression of luciferase, Pit-1 was found to enhance the expression of luciferase. Only one Pit-1 binding site was necessary for enhancement. Analysis by immunoblots of in vitro culture of pituitaries of Sparus aurata showed that several agents, including estradiol, verapamil, and phorbol myristate acetate, had different inhibitory effects on SL and growth hormone released to the culture medium. |
| Databáze: | OpenAIRE |
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