Annealing-modulated nanoscintillators for nonconventional X-ray activation of comprehensive photodynamic effects in deep cancer theranostics
| Autor: | Lun-De Liao, Chia-Hui Chu, Arthur Paldino, Nai-Tzu Chen, Yao-Chen Chuang, Tsung-Sheng Chu, Leu-Wei Lo, Shih-Hsun Cheng, Yu Hsia, Chin-Tu Chen |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
fractionated radiotherapy
medicine.medical_treatment vascular remodeling Medicine (miscellaneous) chemistry.chemical_element Mice Nude Photodynamic therapy X-ray induced radioluminescence 02 engineering and technology 010402 general chemistry 01 natural sciences Oxygen Theranostic Nanomedicine Ionizing radiation chemistry.chemical_compound Mice Cell Line Tumor medicine nanoscintillator Animals Nanotechnology Photosensitizer Yttrium Irradiation Pharmacology Toxicology and Pharmaceutics (miscellaneous) chemistry.chemical_classification Ovarian Neoplasms Reactive oxygen species Photosensitizing Agents Singlet Oxygen Chemistry Singlet oxygen X-Rays Radioluminescence 021001 nanoscience & nanotechnology Silicon Dioxide Xenograft Model Antitumor Assays 0104 chemical sciences radioresistance Photochemotherapy Biophysics Nanoparticles annealing Female 0210 nano-technology Research Paper |
| Zdroj: | Theranostics |
| ISSN: | 1838-7640 |
| Popis: | Photodynamic therapy (PDT), which involves the generation of reactive oxygen species (ROS) through interactions of a photosensitizer (PS) with light and oxygen, has been applied in oncology. Over the years, PDT techniques have been developed for the treatment of deep-seated cancers. However, (1) the tissue penetration limitation of excitation photon, (2) suppressed efficiency of PS due to multiple energy transfers, and (3) insufficient oxygen source in hypoxic tumor microenvironment still constitute major challenges facing the clinical application of PDT for achieving effective treatment. We present herein a PS-independent, ionizing radiation-induced PDT agent composed of yttrium oxide nanoscintillators core and silica shell (Y2O3:Eu@SiO2) with an annealing process. Our results revealed that annealed Y2O3:Eu@SiO2 could directly induce comprehensive photodynamic effects under X-ray irradiation without the presence of PS molecules. The crystallinity of Y2O3:Eu@SiO2 was demonstrated to enable the generation of electron-hole (e--h+) pairs in Y2O3 under ionizing irradiation, giving rise to the formation of ROS including superoxide, hydroxyl radical and singlet oxygen. In particular, combining Y2O3:Eu@SiO2 with fractionated radiation therapy increased radio-resistant tumor cell damage. Furthermore, photoacoustic imaging of tumors showed re-distribution of oxygen saturation (SO2) and reoxygenation of the hypoxia region. The results of this study support applicability of the integration of fractionated radiation therapy with Y2O3:Eu@SiO2, achieving synchronously in-depth and oxygen-insensitive X-ray PDT. Furthermore, we demonstrate Y2O3:Eu@SiO2 exhibited radioluminescence (RL) under X-ray irradiation and observed the virtually linear correlation between X-ray-induced radioluminescence (X-RL) and the Y2O3:Eu@SiO2 concentration in vivo. With the pronounced X-RL for in-vivo imaging and dosimetry, it possesses significant potential for utilization as a precision theranostics producing highly efficient X-ray PDT for deep-seated tumors. |
| Databáze: | OpenAIRE |
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