Infection of Rodents by Orientia tsutsugamushi, the Agent of Scrub Typhus in Relation to Land Use in Thailand
| Autor: | Kittipong Chaisiri, Jean-François Cosson, Serge Morand |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Orientia tsutsugamushi 030231 tropical medicine 030106 microbiology Population Zoology land use land cover lcsh:Medicine Scrub typhus 03 medical and health sciences 0302 clinical medicine Leopoldamys edwardsi medicine Berylmys bowersi education Ribosomal DNA Rattus exulans education.field_of_study General Immunology and Microbiology biology scrub typhus Ecology lcsh:R Public Health Environmental and Occupational Health medicine.disease biology.organism_classification Thailand bacterial infections and mycoses 16S rDNA amplicon sequencing rodents Bandicota savilei Infectious Diseases |
| Zdroj: | Tropical Medicine and Infectious Disease; Volume 2; Issue 4; Pages: 53 Tropical Medicine and Infectious Disease, Vol 2, Iss 4, p 53 (2017) |
| ISSN: | 2414-6366 |
| DOI: | 10.3390/tropicalmed2040053 |
| Popis: | The relationship between land use structures and occurrence of scrub typhus agent, Orientia tsutsugamushi infection in small wild mammals was conducted in three provinces of Thailand: Buriram, Loei, and Nan. Orientia tsutsugamushi detection was performed using 16S ribosomal DNA (rDNA) amplicon sequencing approach using Miseq Illumina platform. In total, 387 animals (rodents and shrews) were examined for the bacterium infection. The 16S rDNA sequences of the bacterium were found in nine animals from Bandicota savilei, Berylmys bowersi, Leopoldamys edwardsi, Rattus exulans, R. tanezumi, and Rattus sp. phylogenetic clade 3, yielding 2.3% infection rate, with two new rodent species infected by the bacterium in Thailand: B. bowersi and L. edwardsi. Using a Generalized Linear Mixed Model (GLMM) and Random Forest analyses for investigating the association between human-land use and occurrence of the bacterium, forest habitat appeared as a strong explicative variable of rodent infection, meaning that O. tsutsugamushi-infected animals were more likely found in forest-covered habitats. In terms of public health implementation, our results suggest that heterogenous forested areas including forest-converted agricultural land, reforestation areas, or fallow are potential habitats for O. tsutsugamushi transmission. Further understanding of population dynamics of the vectors and their hosts in these habitats could be beneficial for the prevention of this neglected zoonotic disease. |
| Databáze: | OpenAIRE |
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