Carbapenem inactivation: a very affordable and highly specific method for phenotypic detection of carbapenemase-producing Pseudomonas aeruginosa isolates compared with other methods

Autor: Behroz Nagili, Reza Ghotaslou, Saber Yousefi, Mohammad Taghi Akhi, Younes Khalili, Hamid Reza Goli, Hossein Samadi Kafil
Rok vydání: 2016
Předmět:
0301 basic medicine
Carbapenem
Hospitalized patients
030106 microbiology
Microbial Sensitivity Tests
Iran
medicine.disease_cause
Polymerase Chain Reaction
Sensitivity and Specificity
beta-Lactam Resistance
beta-Lactamases
law.invention
Microbiology
03 medical and health sciences
Bacterial Proteins
law
Disk Diffusion Antimicrobial Tests
medicine
Escherichia coli
Humans
Pharmacology (medical)
False Positive Reactions
Pseudomonas Infections
Hospital Costs
Hospitals
Teaching
Polymerase chain reaction
Carbapenem resistance
Pharmacology
biology
Pseudomonas aeruginosa
Carbapenemase producing
biology.organism_classification
Phenotype
Virology
Anti-Bacterial Agents
Molecular Typing
Infectious Diseases
Oncology
Carbapenems
Genes
Bacterial
Inactivation
Metabolic
Costs and Cost Analysis
Bacteria
medicine.drug
Zdroj: Journal of chemotherapy (Florence, Italy). 29(3)
ISSN: 1973-9478
Popis: This investigation was undertaken to compare phenotypic and molecular methods for detection of carbapenemase-producing Pseudomonas aeruginosa. A total of 245 non-duplicated isolates of P. aeruginosa were collected from hospitalized patients. Disc diffusion method was used to identify carbapenem-resistant bacteria. Three phenotypic methods, including Modified Hodge Test (MHT), Modified Carba NP (MCNP) test and Carbapenem Inactivation Method (CIM) were used for investigation of carbapenemase production. In addition, polymerase chain reaction (PCR) was used to detect carbapenemase encoding genes. Of 245 P. aeruginosa isolates investigated, 121 isolates were carbapenem-resistant. Among carbapenem-resistant isolates, 40, 39 and 35 isolates exhibited positive results using MHT, MCNP test and CIM, respectively. PCR indicated the presence of carbapenemase genes in 35 of carbapenem-resistant isolates. MHT showed low sensitivity and specificity for carbapenemase detection among P. aeruginosa isolates in comparison to PCR. CIM was most affordable and highly specific than MCNP test compared with the molecular method.
Databáze: OpenAIRE
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