Stimulation of human albumin synthesis and gene expression by growth hormone treatment
| Autor: | L. Råhlén, Pia Essén, Peter J. Garlick, J. Holgersson, Jan Wernerman, H. Barle, Margaret A. McNurlan |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Male medicine.medical_specialty Time Factors Phenylalanine Gene Expression Critical Care and Intensive Care Medicine Albumins Internal medicine Gene expression medicine Humans RNA Messenger Aged Messenger RNA Nutrition and Dietetics medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction business.industry Albumin Metabolism Middle Aged Growth hormone treatment Endocrinology Real-time polymerase chain reaction Cholecystectomy Laparoscopic Gene Expression Regulation Liver Growth Hormone Isotope Labeling Liver biopsy Female business |
| Zdroj: | Clinical Nutrition. 20:59-67 |
| ISSN: | 0261-5614 |
| DOI: | 10.1054/clnu.2000.0158 |
| Popis: | Background and aims: In this study the effects of acute (5 h) and short-term (5 days) GH treatment on albumin synthesis rates in man were investigated and related to changes in the availability of hepatic albumin mRNA. Methods: 30 patients undergoing elective laparoscopic cholecystectomy were randomized into controls (n=10) or GH-treatment (12 U/dose) for 5 h or 5 days (n=10 in each group). Albumin mRNA levels (in liver biopsy specimens) were measured employing a quantitative polymerase chain reaction assay developed specifically for this purpose, whereas albumin synthesis was measured using [2H5]phenylalanine. Results:The fractional synthesis rate of albumin was 6.0±0.9 %/day in the control group and 8.0±1.8 %/day and 8.3±1.7 %/day in the GH-treated groups, respectively (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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