Spoligotyping with pncA sequencing strategy conferring the transmission of multidrug-resistant tuberculosis in Egypt
Autor: | Galal Aggor, Hoida El Naggar, Salah A. Selim, Khaled Al Amry, Suzan Ismail |
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Rok vydání: | 2019 |
Předmět: |
Microbiology (medical)
Silent mutation Adult Male Tuberculosis Adolescent Genotype lcsh:QR1-502 Antitubercular Agents Microbial Sensitivity Tests Biology lcsh:Microbiology DNA sequencing mycobacterium Amidohydrolases Mycobacterium tuberculosis Young Adult Tuberculosis Multidrug-Resistant medicine Humans Mycobacteria growth indicator tube Prospective Studies Aged Genetics spoligotyping multidrug resistant Sputum Genetic Variation Sequence Analysis DNA Middle Aged medicine.disease biology.organism_classification spoligotyping with pnca sequencing Pyrazinamide Bacterial Typing Techniques Multiple drug resistance Infectious Diseases PncA Epidemiological Monitoring Mutation Egypt Female Rifampin Mycobacterium |
Zdroj: | International Journal of Mycobacteriology, Vol 8, Iss 3, Pp 211-217 (2019) |
ISSN: | 2212-554X |
Popis: | Background: This study explored the genetic diversity of Mycobacterium tuberculosis isolates in Egypt by spoligotyping in combination with pncA gene sequencing, spoNC. Methods: First, isolates were selected from 400 isolates positive for M. tuberculosis that referred to Central Labs Ministry of Health and then were subjected to the study analyses. Results: Twenty one isolates were found to be multidrug resistant (MDR) and 29 isolates were sensitive for isonizide (INH) and rifampicine (RIF) after testing by phenotypic drug susceptibility testing (DST) and Mycobacteria Growth Indicator Tube (MGIT). Spoligotyping yielded 45 patterns belonging to seven families that previously reported in neighboring countries such as Iraq, Syria, Iran, and Turkey. While four isolates were orphans. Conclusion: Application of spoNC on obtained spoligotype patterns enhances to reduce the clustering rate. Bejing family the predominant (34%) were subdivided by pncA sequence into three sensitive DST pncA wild type, three MDR-DST isolates showing cys14Arg mutation in pncA, two sensitive DST isolates with pncA Gly97Asp mutation, and three sensitive DST pncAVal128Gly mutation. The next most common CASI_DELHI family (16%) were subdivided by pncA sequencing into CASI_DELHI (st 381, MDR) including two pncA silent mutation ser65ser (tcc > tct) and CASI_DELHI (st26, sensitive) which included six pncA (wild-type) results, and Latin-American-Mediterranean 6 family (6%) all had PncA Gly97Asp mutation. We concluded that spoNC provides good snap shot for MDR surveillance and its country origin and performs early identification of outbreaks in Egypt. |
Databáze: | OpenAIRE |
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