A bioinformatic assay for pluripotency in human cells

Autor:	Bernhard M. Schuldt, Nils Ole Schmidt, Josef B. Aldenhoff, Jeanne F. Loring, Roy Williams, Eirini P. Papapetrou, Dylan Mason, Gulsah Altun, Sandra Danner, Johanna E. Goldmann, Arne Herbst, Franz-Josef Müller, Louise C. Laurent
Rok vydání:	2011
Předmět:	Pluripotent Stem Cells Cell type Rex1 Cellular differentiation Biology Biochemistry Germline Cell Line 03 medical and health sciences 0302 clinical medicine Humans Induced pluripotent stem cell Molecular Biology Cell potency Oligonucleotide Array Sequence Analysis 030304 developmental biology Neurons 0303 health sciences Models Genetic Gene Expression Profiling Computational Biology Cell Differentiation Cell Biology Molecular biology Cell biology Gene expression profiling Gene Expression Regulation Stem cell Software 030217 neurology & neurosurgery Biotechnology
Zdroj:	Nature Methods. 8:315-317
ISSN:	1548-7105 1548-7091
DOI:	10.1038/nmeth.1580
Popis:	Pluripotent stem cells (PSCs) are defined by their potential to generate all cell types of an organism. The standard assay for pluripotency of mouse PSCs is cell transmission through the germline, but for human PSCs researchers depend on indirect methods such as differentiation into teratomas in immunodeficient mice. Here we report PluriTest, a robust open-access bioinformatic assay of pluripotency in human cells based on their gene expression profiles.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da9e4488217061db5e325320583e7f84 https://doi.org/10.1038/nmeth.1580 Zobrazit plný text záznamu Plný text ve formátu PDF