A gene regulatory network anchored by LIM homeobox 1 for embryonic head development
| Autor: | Patrick P.L. Tam, Poh Lynn Khoo, Chi Kin Ip, Mirana Ramialison, Tennille Sibbritt, Guangdun Peng, Joanne X. Shen, Jing-Dong J. Han, Naihe Jing, Nicolas Fossat, Emilie E. Wilkie, Vanessa Jones, Jane Q. J. Sun, Pierre Osteil |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Transcription Genetic LIM-Homeodomain Proteins Gene regulatory network Xenopus 03 medical and health sciences Xenopus laevis Endocrinology Gene expression Genetics Animals Gene Regulatory Networks Gene Transcription factor Embryonic Stem Cells biology Gene Expression Profiling Wnt signaling pathway Genes Homeobox Cell Biology biology.organism_classification Embryonic stem cell Cell biology 030104 developmental biology Germ Cells Homeobox Head Transcription Factors |
| Zdroj: | Genesis (New York, N.Y. : 2000). 56(9) |
| ISSN: | 1526-968X |
| Popis: | Development of the embryonic head is driven by the activity of gene regulatory networks of transcription factors. LHX1 is a homeobox transcription factor that plays an essential role in the formation of the embryonic head. The loss of LHX1 function results in anterior truncation of the embryo caused by the disruption of morphogenetic movement of tissue precursors and the dysregulation of WNT signaling activity. Profiling the gene expression pattern in the Lhx1 mutant embryo revealed that tissues in anterior germ layers acquire posterior tissue characteristics, suggesting LHX1 activity is required for the allocation and patterning of head precursor tissues. Here, we used LHX1 as an entry point to delineate its transcriptional targets and interactors and construct a LHX1-anchored gene regulatory network. Using a gain-of-function approach, we identified genes that immediately respond to Lhx1 activation. Meta-analysis of the datasets of LHX1-responsive genes and genes expressed in the anterior tissues of mouse embryos at head-fold stage, in conjunction with published Xenopus embryonic LHX1 (Xlim1) ChIP-seq data, has pinpointed the putative transcriptional targets of LHX1 and an array of genetic determinants functioning together in the formation of the mouse embryonic head. |
| Databáze: | OpenAIRE |
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