Lung myeloperoxidase as a measure of pulmonary leukostasis in rabbits
| Autor: | K. M. Wu, M. Jay, S. E. Goldblum |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Lung Diseases
Pathology medicine.medical_specialty Time Factors Physiology Lung injury Granulocyte Pulmonary sequestration Sonication Physiology (medical) medicine Animals Hydroxyurea Cyclophosphamide Lung Peroxidase Respiratory Distress Syndrome medicine.diagnostic_test biology Respiratory disease Leukostasis respiratory system medicine.disease respiratory tract diseases Bronchoalveolar lavage medicine.anatomical_structure Streptococcus pneumoniae Myeloperoxidase biology.protein Rabbits Granulocytes |
| Zdroj: | Journal of applied physiology (Bethesda, Md. : 1985). 59(6) |
| ISSN: | 8750-7587 |
| Popis: | Pulmonary leukostasis can be associated with acute lung injury. We studied lung peroxidase activity using myeloperoxidase (MPO) as a granulocyte marker to quantitate pulmonary leukostasis in rabbits. Lungs were homogenized in detergent, freeze-thawed, sonified, and centrifuged, and supernatants were assayed for MPO. Seven extractions were performed, and greater than 80% of cumulative MPO was found in the first three extractions. By use of a three-extraction procedure, the mean lung MPO (delta A X min-1 X g tissue-1) was determined in normal [20.9 +/- 5.2 (SE)], granulocyte-depleted (6.5 +/- 2.0), saline-injected (22.2 +/- 5.6), and pneumococcus (PNC)-challenged (69.7 +/- 10.6) animals. Lung MPO was significantly decreased in granulocyte-depleted compared with normal animals (P less than 0.005) and significantly increased in PNC-challenged compared with saline-injected animals (P less than 0.001). MPO extracted from granulocytes and lungs from normal as well as PNC-challenged animals were all biochemically identical. Lung extract did not inhibit MPO, and no MPO was detected in bronchoalveolar lavage fluid obtained from leukostatic lungs. Lung MPO significantly (P less than 0.01) correlated with intravascular intrapulmonary granulocytes. Determination of lung MPO is a relatively simple quantitative method that can be used to detect pulmonary leukostasis. |
| Databáze: | OpenAIRE |
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