Fecal microbiota transplantation does not alter bacterial translocation and visceral adipose tissue inflammation in individuals with obesity
Autor: | Victor E. A. Gerdes, Abraham S. Meijnikman, Mark Davids, Ömrüm Aydin, Max Nieuwdorp, Guido J. Bakker, Torsten P. M. Scheithauer, Albert K. Groen, Hilde Herrema, Arnold W. J. M. van de Laar, L. Maurits de Brauw, Daniël H. van Raalte, Thomas C. C. Boerlage |
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Přispěvatelé: | VU University medical center, Internal medicine, ACS - Diabetes & metabolism, Amsterdam Gastroenterology Endocrinology Metabolism, AGEM - Endocrinology, metabolism and nutrition, Vascular Medicine, Graduate School, ACS - Atherosclerosis & ischemic syndromes, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Center of Experimental and Molecular Medicine, Paediatric Surgery, Experimental Vascular Medicine |
Rok vydání: | 2021 |
Předmět: |
visceral adipose tissue inflammation
Endocrinology Diabetes and Metabolism Adipose tissue Chromosomal translocation Inflammation Type 2 diabetes Gut flora Insulin resistance medicine bacterial translocation Internal medicine Nutrition and Dietetics gut microbiota biology business.industry fecal microbiota transplantation Original Articles biology.organism_classification medicine.disease RC31-1245 Obesity Immunology Original Article medicine.symptom Metabolic syndrome business |
Zdroj: | Obesity Science & Practice Obesity Science and Practice. Wiley-Blackwell Obesity Science & Practice, Vol 8, Iss 1, Pp 56-65 (2022) Bakker, G J, Meijnikman, A S, Scheithauer, T P, Davids, M, Aydin, Ö, Boerlage, T C C, de Brauw, L M, van de Laar, A W, Gerdes, V E, Groen, A K, van Raalte, D H, Herrema, H & Nieuwdorp, M 2021, ' Fecal microbiota transplantation does not alter bacterial translocation and visceral adipose tissue inflammation in individuals with obesity ', Obesity Science and Practice . https://doi.org/10.1002/osp4.545 |
ISSN: | 2055-2238 |
DOI: | 10.1002/osp4.545 |
Popis: | Aims Visceral adipose tissue inflammation is a fundamental mechanism of insulin resistance in obesity and type 2 diabetes. Translocation of intestinal bacteria has been suggested as a driving factor for the inflammation. However, although bacterial DNA was detected in visceral adipose tissue of humans with obesity, it is unclear to what extent this is contamination or whether the gut microbiota is causally involved. Effects of fecal microbiota transplantation (FMT) on bacterial translocation and visceral adipose tissue inflammation in individuals with obesity and insulin resistance were assessed. Material and Methods Eight individuals with clinically severe obesity (body mass index [BMI] >35 kg/m2) and metabolic syndrome received lean donor FMT 4 weeks prior to elective bariatric surgery. The participants were age‐, sex‐, and BMI‐matched to 16 controls that underwent no fecal transplantation. Visceral adipose tissue was collected during surgery. Bacterial translocation was assessed by 16S rRNA gene sequencing of adipose tissue and feces. Pro‐inflammatory cytokine expression and histopathological analyses of visceral adipose tissue were performed to assess inflammation. Results Fecal microbiota transplantation significantly altered gut microbiota composition. Visceral adipose tissue contained a very low quantity of bacterial DNA in both groups. No difference in visceral bacterial DNA content between groups was observed. Also, visceral expression of pro‐inflammatory cytokines and macrophage infiltration did not differ between groups. No correlation between inflammatory tone and bacterial translocation was observed. Conclusions Visceral bacterial DNA content and level of inflammation were not altered upon FMT. Thus, bacterial translocation may not be the main driver of visceral adipose tissue inflammation in obesity. |
Databáze: | OpenAIRE |
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