Kinetics of Thymocyte Subset Development and SelectionRevealed by Cyclosporin A Treatment
| Autor: | Russell D. J. Huby, Ray Hicks, Lindsey K. Goff |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
medicine.medical_specialty CD8 Antigens Receptors Antigen T-Cell alpha-beta Cellular differentiation Immunology Kinetics Thymus Gland Biology Flow cytometry Mice Cyclosporine A T-Lymphocyte Subsets Internal medicine Cyclosporin a medicine Animals CD45 Lymphocyte Count development autoreactivity Mice Inbred BALB C medicine.diagnostic_test apoptosis thymocyte Cell Differentiation CsA Growth Inhibitors Cell biology Mice Inbred C57BL Thymocyte Endocrinology Mature Thymocyte Apoptosis kinetics CD4 Antigens Cyclosporine Female lcsh:RC581-607 Immunosuppressive Agents CD8 Research Article Developmental Biology |
| Zdroj: | Clinical and Developmental Immunology, Vol 4, Iss 2, Pp 117-126 (1995) Developmental Immunology |
| ISSN: | 1740-2530 1740-2522 |
| Popis: | Cyclosporin A (CsA) inhibits the development of mature thymocytes from their CD4+CD8+precursors, but may allow autoreactive cells to mature. Using 3-color flow cytometry, we have followed the progressive development of thymocytes, including potentially autoreactive cells, during CsA treatment. Numbers of CD4+CD8+CD3highthymocytes dropped immediately, suggesting that the generation of these mature thymocyte precursors, normally dependent upon positive selection, was inhibited by CsA. Numbers of CD4-CD8+thymocytes also declined rapidly, but CD4-CD8+thymocytes were unaffected lfor 2 days, suggesting that the mature single-positive subsets are not symmetrically derived from a common GsA-sensitive precursor. An exceptional subset of CD8 SP thymocytes, expressing CD45RA, did not respond to CsA for about 10 days, indicating that they are distantly derived from a CsA-sensitive precursor. Apoptosis of TCR-Vβ3+thymocytes caused byMtυ-6, quantified according to the down-regulation of CD4 and CD8 on immature thymocytes, was partially inhibited by CsA, to maximal effect within 24 hours. This did not, however, facilitate their development into mature thymocytes. |
| Databáze: | OpenAIRE |
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