NOTEExpression of prolactin receptors and regulation of cell proliferation by prolactin, corticotropin-releasing factor, and corticosterone in a neuroblastoma cell line
| Autor: | Y N Mohammad, P M Ingleton, David A. Lovejoy, Maria G. Castro, L Wang, M Perone |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
endocrine system
medicine.medical_specialty Corticotropin-Releasing Hormone Receptors Prolactin Molecular Sequence Data Neuropeptide Biochemistry Mice Neuroblastoma chemistry.chemical_compound Corticosterone Internal medicine medicine Animals RNA Messenger Receptor Molecular Biology Microscopy Confocal Base Sequence Cell growth Cell Biology medicine.disease Prolactin Endocrinology chemistry Cell culture Cell Division hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Biochemistry and Cell Biology. 80:475-482 |
| ISSN: | 1208-6002 0829-8211 |
| DOI: | 10.1139/o02-036 |
| Popis: | The aetiology of neuroblastoma remains obscure, although a number of neuropeptides have been implicated in its pathogenesis. Using the mouse neuroblastoma cell line Neuro2a as a model, we have investigated the mitogenic actions of prolactin (PRL) and two hypothalamopituitaryadrenal stress axis hormones, corticotropin-releasing factor (CRF) and corticosterone. Using established polyclonal PRL receptor antisera with immunofluorescence cytochemistry, we show that the Neuro2a cells possess immunoreactive forms of both the long and short forms of the receptor. PRL and CRF were effective as mitogens in Neuro2a cell cultures, where a 107M concentration of PRL or CRF elicited a two-fold increase in the numbers of cells after 72 h (p < 0.0001). Corticosterone, however, attenuated their proliferation. These data suggest that prolactin may act to increase the proliferation and regulation of neuroblastomas and that the effects of PRL may be modified by hypothalamopituitaryadrenal hormones.Key words: cell proliferation, mitogens, neuroblasts, stress, cancer. |
| Databáze: | OpenAIRE |
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