IgE-reactivity pattern of tomato seed and peel nonspecific lipid-transfer proteins after in vitro gastrointestinal digestion
| Autor: | Sara Benedé, Elena Molina, Francisca Gómez, Cristobalina Mayorga, Miguel Gonzalez, Laura Martín-Pedraza, Mónica Martínez-Blanco, Mayte Villalba, Natalia Blanca-López, Cristina Bueno-Díaz, Javier Cuesta-Herranz |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Instituto de Salud Carlos III, European Commission, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España) |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
biology Stomach 010401 analytical chemistry food and beverages General Chemistry medicine.disease_cause Immunoglobulin E medicine.disease 01 natural sciences In vitro 0104 chemical sciences chemistry.chemical_compound Allergen medicine.anatomical_structure Biochemistry chemistry Phosphatidylcholine medicine biology.protein General Agricultural and Biological Sciences Digestion Plant lipid transfer proteins Anaphylaxis 010606 plant biology & botany |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | The influence of gastrointestinal digestion on the immunological properties of three different nonspecific lipid-transfer proteins (nsLTPs) described in tomato fruit has been assessed using an in vitro system mimicking the stomach and intestine digestion conditions. Tomato peel/pulp nsLTP, Sola l 3, was degraded after digestion, although the immunoglobulin E (IgE) recognition of intact protein and a 10 kDa band were still observed after 30 min of duodenal digestion in the presence of phosphatidylcholine. The tomato seed nsLTP, Sola l 7, showed a higher stability than the other seed allergen, Sola l 6, during digestion. Sola l 7 showed an IgE immunoreactive 6.5 kDa band in immunoblotting analysis, retaining up to 7% of its IgE-binding capacity in inhibition ELISA test after 60 min of duodenal digestion and keeping intact its ability to activate basophils after digestion. These results suggest that the tomato seed allergen Sola l 7 might be considered as an important allergen in the induction of allergic responses to tomato due to its high stability against gastrointestinal digestion. This work was supported by grants SAF2017-86483-R from the Ministerio de Economía y Competitividad and PI13/00928 Instituto de Salud Carlos III (ISCIII), ISCIII cofounded by Fondo Europeo de Desarrollo Regional – FEDER for the Thematic Networks and Co-operative Research Centres; RIRAAF RD16/0006/0024 and ARADyAL (RD16/0006/0001; RD16/0006/0013; RD16/0006/0014); and EAACI Exchange Research Fellowship 2016. The authors would like to thank the excellent technical support of Sara Abián. S.B. was supported by a Juan de la Cierva Incorporación contract from the Spanish Ministerio de Ciencia, Innovación y Universidades. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|