Sardine roe as a source of lipids to produce liposomes
| Autor: | Ana R. Costa-Pinto, Marta Guedes, Nuno M. Neves, Rui L. Reis, Joana Moreira-Silva, Helena Lage Ferreira, Virgínia M.F. Gonçalves, Maria Elizabeth Tiritan |
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| Přispěvatelé: | Universidade do Minho, Veritati - Repositório Institucional da Universidade Católica Portuguesa |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0206 medical engineering
Biomedical Engineering Bligh and Dyer 02 engineering and technology methyl-tent-butyl ether fatty acids Biomaterials chemistry.chemical_compound Animals Humans Food science Phospholipids Liposome Science & Technology Chemistry Methyl- tert-butyl ether Sardine Fishes sardine roe 021001 nanoscience & nanotechnology Cytocompatibility 020601 biomedical engineering Seafood Liposomes Fish 0210 nano-technology methyl-tert-butyl ether human activities Methyl tert-butyl ether |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Sea-derived materials have promising applications in the medical, pharmaceutical, and biotechnological fields. Fish roe, for example, is a highly nutritional product, presenting diverse beneficial effects on human health. Therefore, this work explored extracts of sardine (Sardina pilchardus) roe, due to the well-known health benefits of this fish, to produce novel and promising delivery systems. After morphological, histological, and histochemical characterizations of sardine roe, their lipids were extracted using two different approaches, namely, Bligh and Dyer (BD) and methyl-tert-butyl ether (MTBE) methods. Gas chromatography/mass spectrometry analyses demonstrated that lipid extracts contain several fatty acids, such as Ï 3 polyunsaturated fatty acids. The lipids, especially phospholipids, were used to produce multilamellar liposomes (MLVs). These delivery systems presented size heterogeneity, a negative surface charge, and the ability to control the release of the encapsulated anti-inflammatory drug, namely, celecoxib. Biological assays indicated that MLVs produced with MTBE lipidic extracts presented a better cytocompatibility than those obtained by the BD method. This can be further improved if the lipid extracts are processed by chemical extraction. Therefore, sardine roe-derived lipids can produce drug-delivery systems with the potential to be applied in the biomedical field. The authors acknowledge thefinancial support from FCT/MCTES and the FSE/POCH, PD/169/2013, for the PhDgrant (PD/BD/113795/2015) attributed to Marta Guedes andthe Post-doc Grant attributed to Ana R. Costa-Pinto (SFRH/BPD/90332/2012), and the SPARTAN project (PTDC/CTM-BIO/4388/2014). They thank the localfisherman forthe donation of the samples, Narcisa Bandarra and CristinaNunes for their support in the histological analysis, and the staff from the Centre of Biological Engineering of the University of Minho for their collaboration in the fatty acids analysis. |
| Databáze: | OpenAIRE |
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