X-ray micro-tomography for investigations of brain tissues on cellular level
| Autor: | Simone E. Hieber, Peter Thalmann, Georg Schulz, Gabriel Schweighauser, Christos Bikis, Jürgen Hench, Marie-Christine Zdora, Alexander Hipp, Hans Deyhle, Bert Müller, Irene Zanette, Anna Khimchenko |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science business.industry media_common.quotation_subject Resolution (electron density) X-ray Synchrotron radiation law.invention 03 medical and health sciences Interferometry 030104 developmental biology Optics Beamline Optical microscope law Contrast (vision) Tomography business media_common |
| Zdroj: | Developments in X-Ray Tomography X |
| ISSN: | 0277-786X |
| Popis: | X-ray imaging in absorption contrast mode is well established for hard tissue visualization. However, performance for lower density materials is limited due to a reduced contrast. Our aim is three-dimensional (3D) characterization of micro-morphology of human brain tissues down to (sub-)cellular resolution within a laboratory environment. Using the laboratory-based microtomography (μCT) system nanotom m (GE Sensing and Inspection Technologies GmbH, Wunstorf, Germany) and synchrotron radiation at the Diamond-Manchester Imaging Branchline I13-2 (Diamond Light Source, Didcot, UK), we have acquired 3D data with a resolution down to 0.45 μm for visualization of a human cerebellum specimen down to cellular level. We have shown that all selected modalities, namely laboratory-based absorption contrast micro-tomography (LBμCT), synchrotron radiation based in-line single distance phase contrast tomography (SDPR) and synchrotron radiation based single-grating interferometry (GI), can reach cellular resolution for tissue samples with a size in the mm-range. The results are discussed qualitatively in comparison to optical microscopy of haematoxylin and eosin (HE) stained sections. As phase contrast yields to a better data quality for soft tissues and in order to overcome restrictions of limited beamline access for phase contrast measurements, we have equipped the μCT system nanotom m with a double-grating phase contrast set-up. Preliminary experimental results of a knee sample consisting of a bony part and a cartilage demonstrate that phase contrast data exhibits better quality compared to absorption contrast. Currently, the set-up is under adjustment. It is expected that cellular resolution would also be achieved. The questions arise (1) what would be the quality gain of laboratory-based phase contrast in comparison to laboratory-based absorption contrast tomography and (2) could laboratory-based phase contrast data provide comparable results to synchrotron radiation based phase contrast data. |
| Databáze: | OpenAIRE |
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