Cell surface heparan sulfate proteoglycans are involved in the extracellular Hsp90-stimulated migration and invasion of cancer cells
| Autor: | Y. Y. Skarga, A. V. Snigireva, V. V. Vrublevskaya, Oleg S. Morenkov |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell Biochemistry Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement Cell Line Tumor Extracellular medicine Animals Humans Neoplasm Invasiveness HSP90 Heat-Shock Proteins Protein kinase B Original Paper Heparinase Cell Membrane Cell Biology Heparan sulfate Cell biology 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Cancer cell Cattle HT1080 Heparan Sulfate Proteoglycans Intracellular |
| Zdroj: | Cell Stress and Chaperones. 24:309-322 |
| ISSN: | 1466-1268 1355-8145 |
| DOI: | 10.1007/s12192-018-0955-5 |
| Popis: | The extracellular heat shock protein 90 (Hsp90) is known to participate in cell migration and invasion. Recently, we have shown that cell surface heparan sulfate proteoglycans (HSPGs) are involved in the binding and anchoring of extracellular Hsp90 to the plasma membrane, but the biological relevance of this finding was unclear. Here, we demonstrated that the digestion of heparan sulfate (HS) moieties of HSPGs with a heparinase I/III blend and the metabolic inhibition of the sulfation of HS chains by sodium chlorate considerably impair the migration and invasion of human glioblastoma A-172 and fibrosarcoma HT1080 cells stimulated by extracellular native Hsp90. Heparin, a polysaccharide closely related to HS, also reduced the Hsp90-stimulated migration and invasion of cells. Phorbol 12-myristate 13-acetate, an intracellular inducer of cell motility bypassing the ligand activation of receptors, restored the basal migration of heparinase- and chlorate-treated cells almost to the control level, suggesting that the cell motility machinery was insignificantly affected in cells with degraded and undersulfated HS chains. On the other hand, the downstream phosphorylation of AKT in response to extracellular Hsp90 was substantially impaired in heparinase- and chlorate-treated cells as compared to untreated cells. Taken together, our results demonstrated for the first time that cell surface HSPGs play an important role in the migration and invasion of cancer cells stimulated by extracellular Hsp90 and that plasma membrane-associated HSPGs are required for the efficient transmission of signal from extracellular Hsp90 into the cell. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12192-018-0955-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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